Abstract:

The Pleurotus ostreatus were used as the raw material for extracting polysaccharide by means of smashing extractor, ultrasonic extraction, ultrasonicassisted complex enzyme extraction. The results indicate the  optimal extraction conditions of the smashing extractor were extraction temperature 90 ℃, extraction rate 11 350 r/min, extraction time 20 min, extraction 3 times, and the yield of polysaccharide extracted was 11.20%. The optimal extraction conditions of ultrasonic extraction were extraction temperature 60 ℃, extraction time 60 min, ultrasonic power 450 W, extraction 3 times, and the yield of polysaccharide extracted was 9.75%. The optimal extraction conditions of ultrasonicassisted complex enzyme extraction were extraction temperature 30 ℃, extraction time 60 min, ultrasonic power 350 W, extraction pH 5, the yield of polysaccharide extracted was 1205%. In comprehensive consideration of the yield of polysaccharide extracted, saving energy, simple operation, the smashing extractor was considered as an ideal method.

Key words: Pleurotus ostreatus polysaccharide, smashing extraction; ,  ultrasonic extraction, ultrasonicassisted complex enzyme;orthogonal array design