Abstract:

According to the SQS gene of Panax ginseng, the authors designed a sense primer and an antisense primer to construct a SQS interference expression vector. The ginseng callus was transformed by agrobacteriummediated methods, and the selected resistant callus was assayed by PCR and realtime PCR, and the ginsenosides of resistant callus was analyzed by HPLC. Compared with the nontransformation ginseng callus, the SQS gene’s expression of positive callus decreased, and the ginsenosides also had certain changes. SQS is a critical biosynthetic enzyme of ginsenoside. To inhibit the expression of SQS can mediate the change of the content of the ginsenosides of ginseng.

Key words: Panax ginseng; , interference vector, SQS gene, callus of Panax ginseng