Abstract: Cyt c/Au/AAO/PySSPy electrodes were made by immobilizing Cyt c on PySSPy modified gold electrodes covered with AAO templates. The cyclic voltammgram of Cyt c/Au/AAO/PySSPy films shows a pair of well-defined and nearly reversible peaks at about 0.059 V(vs. Ag/AgCl) in pH 6.8 PBS buffers, being the chara cteristic peaks of the Cyt c heme Fe(Ⅲ)/Fe（Ⅱ）redox couples. In the microenvironm ent of the AAO/PySSPy film, the electron transfer between Cyt c and the electrode was greatly enhanced. The soret absorption band positions suggest that Cyt c retained its native conformation in AAO templates. The hydrogen peroxide was cat alytically reduced at Cyt c/Au/AAO/PySSPy electrodes.

Key words: nano-alumina, cytochrome c, direct electrochemistry, electrochemical catalysis