Abstract:

To establish a method to determine the concentration of notoginsenoside C in plasma, a variety of methods were examined in the experiment to try to detect the mass concerntration of notoginsenoside C in plasma, and finally the method of extraction with methanol with protopanaxadiol as the internal standard was confirmed the best. The RPHPLC method was estabilished and the conditions were ODS C18 column (4.6 mm×250 mm, 5 μm), the mobile phase of methanolwater (V(methonol) ∶V(water)=90 ∶10), flow rate of 1.0 mL/min, and detection wavelength 203 nm. The retention time of notoginsenoside C and that of protopanaxadiol were respectively 136 min and 23.6 min. The peaks of samples were seperated well from each other without being interrupted by other components peaks. RSD of daily precision was less than 4.38%, RSD  of interday precision  was 5.22%, and the method recovery was in a range from 95.2% to 99.1%.

Key words: notoginsenoside C, plasma drug concentration, reverse phase high performance liquid (RP-HPLC)