Abstract:

Two rabbits were immunized six times with the whole integrinlinked kinaseassociated phosphatase (ILKAP) protein as antigen by intramuscular and intravenous injections, and the antiserum against ILKAP was obtained. Then, antiILKAP polyclonal antibody was prepared via Protein A agrose column. Western blot analysis shows that the antiILKAP polyclonal antibody had higher specificity, and the antibody titer came to 1 ∶1 600 via indirect enzymelinked immunosorbent assay (indirect ELISA). Immunofluorescence analysis demonstrates that the prepared ILKAP polyclonal antibody had a good application in cell researches.

Key words: integrinlinked kinaseassociated phosphatase (ILKAP), antiserum, antibody, titer, specificity