Abstract:

RACE (rapidamplification of cDNAends) technique was used to clone Na⁺/H⁺ antiporter gene from Glycine max L Merr with the total RNA as the template. This gene was linked to the plasmid PBI121 and the expression vector PBI121NHX3 was constructed. The results show its ORF（open reading frame）consists of 1 503 bp, which can be deduced to encode 501 amino acids. It was found that predicted amino acid sequence had an identification of 72%— 94% compared with amino acid sequence from other ten plant species and it had a typical structural domain of the monovalent cation(proton) antiporter. The gene was named GmNHX3, (GenBank accession number: JN872904). Genetic transformation vector of PBI121NHX3 was constructed successfully by identification method of PCR and restriction enzyme digestion.

Key words: Glycine max L Merr, Na⁺/H⁺ antiporter;  construction vector