Abstract:

To supply the required large amounts of functional recombinant plant expression vectors, a series of general plant binary expression vectors with the CaMV35S and the rd29A promoter mediating multiple cloning sites (MCS: SacⅠ, KpnⅠ, SmaⅠ, BamⅠ, XbaⅠ, SalⅠ, Pst
Ⅰ) were constructed successfully, on the basis of pCHF3, pCAMBIA1301, pCAMBIA3300 and pCAMBIA3301 vectors, and a set of applicative binary expression vectors utilized for subcellular localization by the fusion of MCS and eGFP with these two promoters were also constructed. Subsequently, the universal highthroughput and expensesaving gene expression system was established for the scan and functional characterization of a large number of candidate genes.

Key words: plant binary expression vector, CaMV 35S, rd29A, multple clonging sites(MCS), enhanced green fluorescent protein (eGFP)