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Extraction Optimized of Kaempferol and Biochemical Properties
ZHANG Lei, WANG Luyao, FU Xueqi, ZHAO Wanzhu, HAO Jingwei, WANG Xinying, JI Qiuyan
Journal of Jilin University Science Edition. 2022, 60 (1):
175-0181.
The extraction conditions of kaempferol were optimized by a two-phase acid hydrolysis method, and the effects of acid concentration, extraction time and solid-liquid ratio on the extraction yield of kaempferol were investigated by a single factor experiment combined with high performance liquid chromatography (HPLC). The response surface Box-Behnken experiment was used to optimize the extraction process of kaempferol. The experimental results show that the optimum extraction conditions are the concentration of hydrochloric acid of 6.1×10-2 mmol/L, the extraction time of 12.87 min, the solid-liquid ratio of 1∶137.46, and the extraction yield is 1.11%. Kaempferol has a certain antioxidant effect, and has certain scavenging ability on superoxide free radical (·O2-) and 1,1-diphenyl-2-picrylhydrazyl radical 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH·), especially on DPPH·, and the scavenging ability increases with the increase of concentration.
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