Abstract:

In order to make the suspended microspheres to be detected separately and monostichously, based on the technical principles of flow cytometry, a kind of microfluidic focusing chip was designed. The focusing chip was simulated and designed by using software Intellisuite. This PDMSbased microfluidic chip was produced by casting method,  adopting a sealing method as contact pins, which greatly improved the yield of chips. Through the experiment,  the microfludic focusing chip is fabricated, and is applied to the testing realizing the suspended 10 μm diameter microspheres ordered separate and monostichous.

Key words: focusing, microfluidic chip, biological detection