Abstract:

To predict the siRNA(Small interfering Ribonucleic Acid) efficiency accurately, by the statistical analysis of 2 431 siRNA,we found a reliable new method of forecasting.We selected 19 sequence characteristics related to siRNA efficiency with statistical method and made out a clustering method for siRNA inhibiting efficiency level with these characteristics,the correct rate is up to 81.1%. This method provides orientation for high efficient siRNA design.

Key words: ribonucleic acid interference(RNAi), prediction of interference efficiency, sequence characteristic, statistical analysis