抗诱变中草药的正交实验

doi:吉林省计划经济委员会资助课题

抗诱变中草药的正交实验

刘洋¹，刘冰^1*，祝威²

1. 吉林大学基础医学院医学遗传学教研室,吉林　长春130021;2.吉林大学第一医院耳鼻咽喉-头颈外科, 吉林　长春130021

收稿日期:2004-04-02 修回日期:1900-01-01 出版日期:2004-11-28 发布日期:2004-11-28
通讯作者: 刘冰

Orthogonal experiment of anti-mutagenesisChinese herbal medicines

LIU Yang¹, LIU Bing^1*, ZHU Wei²

1.Department of Medical Genetics, School of Basic Medical Sciences, Jilin University, Changchun 130021,China;2.Department of Otorhinolaryngology and Head-Neck Surgery, First Hospital, Jilin University,Changchun 130021,China

Received:2004-04-02 Revised:1900-01-01 Online:2004-11-28 Published:2004-11-28
Contact: LIU Bing

摘要/Abstract

摘要： 目的：通过正交实验确定抗诱变中草药人参、五加皮、猪苓和甘草保护染色体稳定性的最佳配伍方案。方法：采用徐道觉（Hsu TC）建立的以博来霉素（BLM）为诱变剂的诱变剂敏感性实验方法，将上述4味中草药药量取3个水平：大剂量（常用量2倍）、常用剂量及零水平；把此4味中药随机分组,人参： C、五加皮：D、猪苓：A、甘草：B；分别在培养的人外周血淋巴细胞中加入不同剂量配比的人参、五加皮、猪苓和甘草，比较各剂量配比中草药干预后的染色体断裂率（b/c：每分裂相染色体的断裂数），观察它们的抗诱变效果。结果：人参、五加皮、甘草和猪苓的极差分别为16、14、 9和6，配伍中4味中草药主次顺序依次为人参、五加皮、猪苓、甘草；最佳配比A₁B₂C₁D₂。结论：采用最佳染色体稳定剂配比方案(A₁B₂C₁D₂)进行药物配伍可以最大限度地抵抗诱变剂BLM的作用，增强染色体的稳定性。

关键词: 中草药, 中药配伍

Abstract: Objective To obtain the best compatibility prescription to protect the stability of chromosome in which the Chinese herbal medicines had the role of anti-mutagesis including ginseng,slenderstyle acanthopanax root-bark, agaric, and Glycyrrhiza uralensis with orthogonal experiment. Methods The mutagen-sensibity experiment created by Hsu TC in which Bleomycin (BLM) was used as mutagen was adopted. The dosages of the above-mentioned Chinese herbal medicines in the experiment were applied in 3 levels: large dosage (double commonly used dosage), commonly used dosage and zero. The traditional Chinese medicines were randomly divided into four groups:A,B,C, and D. Ginseng was represented by C; slenderstyle acanthopanax root-bark was represented by D; agaric by A and Glycyrrhiza uralensis by B. The Chinese herbal medicines composed of different ratio with ginseng,slenderstyle acanthopanax root-bark, agaric, and Glycyrrhiza uralensis,respectively, were added into cultured lymphocytes of human peripheral blood.The chromosome breakage rates (b/c: chromosome breakage number per cell) intervened by the Chinese herbal medicines composed of different dosage ratio were compared and the anti-mutagesis effects were observed. Results The ranges of ginseng,slenderstyle acanthopanax root-bark ,agaric, and Glycyrrhiza uralensis was 16,14,9, and 6,respectively;the importance of the 4 kinds of Chinese herbal medicines in the compatibility prescription in order was ginseng,slenderstyle acanthopanax root-bark, agaric, and Glycyrrhizia uralensis; the best compatibility prescription was A₁B₂C₁D₂. Conclusion The best compatibility prescription to protect the stability of chromosome is A₁B₂C₁D₂.It is the greatest resistance to anti-mutagenic agents and reinforcement of chromosome stability to adopt above-mentioned dosage ratio.

Key words: drugs, Chinese herbal , drugs, Chinese herbal compatibility prescription

中图分类号:

R394.6

刘洋，刘冰，祝威. 抗诱变中草药的正交实验[J]. J4, 2004, 30(6): 882-884.

LIU Yang, LIU Bing, ZHU Wei. Orthogonal experiment of anti-mutagenesisChinese herbal medicines[J]. J4, 2004, 30(6): 882-884.