全脑缺血再灌注血管性痴呆大鼠海马和丘脑NT-3及FGF的动态变化

doi:吉林省科技厅资助课题（200001）

全脑缺血再灌注血管性痴呆大鼠海马和丘脑NT-3及FGF的动态变化

李爱丽¹，周钢²，范恩学³，刘娜¹

1. 吉林大学第二医院神经内科，吉林长春130041； 2. 吉林省长春市中级人民法院，吉林长春130061；3. 吉林大学第二医院普通外科，吉林长春130041

收稿日期:2005-04-28 修回日期:1900-01-01 出版日期:2006-01-28 发布日期:2006-01-28

Dynamic changes of NT-3 and FGF in hippocampus and thalamusafter cerebral ischemia reperfusion in vascular dementia rats

LI Ai-li¹，ZHOU Gang², FAN En-xue³, LIU Na¹

1. Department of Neurology, Second Hospital, Jilin University, Changchun 130041, China; 2. Changchun Intermediate People′s Court, Changchun 130061, China; 3. Department of General Surgery, Second Hospital, Jilin University, Changchun 130041,China

Received:2005-04-28 Revised:1900-01-01 Online:2006-01-28 Published:2006-01-28

摘要/Abstract

摘要： 目的：探讨血管性痴呆与神经营养因子-3(NT-3)、成纤维细胞因子（FGF）之间的关系。方法：Wistar老龄大鼠雌雄随机分为假手术组和实验组，实验组包括全脑缺血15 min组及缺血15 min再灌注1 h、6 h、2 d、4 d、9 d组，每组5只。用免疫组化ABC法检测缺血再灌注不同时相海马和丘脑NT-3及FGF表达。结果：正常老龄大鼠海马和丘脑均含有少量NT-3及FGF；缺血再灌注1 h～4 d，海马 NT-3表达迅速而持续增强，与对照组比较差异有显著性（P<0.05），缺血再灌注9 d时减弱至对照组水平（P>0.05）；缺血再灌注6 h～2 d，海马FGF表达增强，与对照组比较差异有显著性(P<0.05）；缺血再灌注4 d时其表达进一步增强（P<0.01），随后其表达锐减，与对照组比较差异无显著性（P>0.05）；缺血再灌注2 d，丘脑NT-3和FGF 表达开始增强，与对照组比较差异有显著性（P<0.05），随后二者表达均减弱，与对照组比较差异无显著性（P>0.05）。结论：海马对缺血损伤具有反应快、作用持久的NT-3和FGF保护机制，丘脑缺乏良好的NT-3和FGF保护机制。

关键词: 血管性, 神经营养因子3, 成纤维细胞生长因子, 脑缺血, 大鼠, Wistar

Abstract: Objective To investigate the correlations between vascular dementia and neurotrophin-3(NT-3)，fibroblast growth factor(FGF). Methods Female and male Wistar senile rats were divided into the control group and the operation groups at random. The operation groups consisted of the cerebral ischemia 15 min group and ischemia 15 min reperfusion groups in which the rats were reperfused at 0，1st，6th hour and on 2nd，4th，9th day with five rats per group. The expressions of NT-3 and FGF in hippocampus and thalamus were examined in different periods of ischemia-reperfusion with ABC method of immunohistochemistry. Results There was a little expressions of NT-3 and FGF in hippocampus and thalamus of normal senile rats;the expression of NT-3 in hippocampus increased quickly and steadily from 1 h to 4 d after ischemia-reperfusion; there were signifi cant differences compared with control group （P<0.05）. The expression of NT-3 in hippocampus reduced on 9th day after ischemia-reperfusion.There were no differences compared with control group （P>0.05）. The expression of FGF in hippocampus increased from 6 h to 2 d after ischemia-reperfusion（P<0. 05）, the expression of FGF increased further on 4th day （P<0.01）. Then it reduced sharply, there were no differences compared with control group （P>0.05）. FGF in thalamus began to increase on 2nd day after ischemia-reperfusion and there were significant differences compared with control group （P <0.05）. Then the expressions of NT-3 and FGF in thalamus decreased, there were no differences compared with control group （P>0.05）. Conclusion Hippocampus has the protective mechanism of NT-3 an d FGF against ischemia injury which is produced quickly and has endurable effects, but thalamus lacks the good protective mechanism of NT-3 and FGF.

Key words: vascular, neurotrophin 3, fibroblast growth factors, brain ischemia, rats, Wistar

中图分类号:

R363.21 A

李爱丽，周钢，范恩学，刘娜. 全脑缺血再灌注血管性痴呆大鼠海马和丘脑NT-3及FGF的动态变化[J]. J4, 2006, 32(1): 57-4.

LI Ai-li，ZHOU Gang, FAN En-xue, LIU Na. Dynamic changes of NT-3 and FGF in hippocampus and thalamusafter cerebral ischemia reperfusion in vascular dementia rats[J]. J4, 2006, 32(1): 57-4.

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