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• 临床研究 • 上一篇    下一篇

精液白细胞与精子形态学参数的关系

李 哲1,王春莲2,王瑞雪1,刘睿智1*,马玉涵1,江 露1   

  1. 1. 吉林大学基础医学院细胞生物学教研室,吉林 长春130021; 2. 青岛大学附属医院生殖医学中心,山东 青岛266003
  • 收稿日期:2004-09-06 修回日期:1900-01-01 出版日期:2006-01-28 发布日期:2006-01-28
  • 通讯作者: 刘睿智

Relationship between leukocyte concentrationin semen and sperm morphological parameters

LI Zhe1,WANG Chun-lian2, WANG Rui-xue1,LIU Rui-zhi1*, MA Yu-han1,JIANG Lu1   

  1. 1. Department of Cell Biology,School of Basic Medical Sciences, Jilin University,Changchun 130021, China; 2. Department of Reproductive Medicine Center, Affiliated Hospital of Qingdao University, Qingdao 266003, China
  • Received:2004-09-06 Revised:1900-01-01 Online:2006-01-28 Published:2006-01-28
  • Contact: LIU Rui-zhi

摘要: 目的:探讨精液白细胞与精子形态学参数之间关系。 方法:对278例不育患者采用精子形态检测系统下人工修正方法进行精子形态分析,采用精液白细胞计数法检测精液白细胞,采用改良巴氏染色法分析精子顶体形态。结果:白细胞精子症组形态正常精子率和顶体完整率均低于非白细胞精子症组(P<0.05);形态正常精子率与精液白细胞浓度呈负相关关系(r=-0.243,P<0.01);顶体完整率与精液白细胞浓度亦呈显著负相关关系( r=-0.248,P<0.01)。结论:精液白细胞增多可能影响精子的形态和顶体的完整。

关键词: 男性, 精液, 白细胞, 精子形态, 顶体

Abstract: Objective To investigate the relationship between WBC concentration in semen and sperm morphological parameters. Methods Semen samples were collected from 278 infertile men. WBC in semen was detected through WBC count by microscope.Sperm morphology was evaluated using artificially modified method by Automa ted Sperm Morphology Analyzer.Acrosomal morphology was analysed with modified P apanicolaou staining.Results The percentage of morphologically normal sperm and t he rate of acrosomal intactness in leukocytospermic group were both significantl y lower than those in nonleukocytospermic group(P<0.05).The WBC concentration in semen had significantly negative correlation with the percentage of morphologically normal sperm and the rate of acrosomal intactness(r=-0.243, P<0.01; r=-0.248, P<0.01). Conclusion Excessively high WBC concentration in semen could affect morphology of sperm and acrosomal intactness.

Key words: male, semen, leukocytes, sperm morphology, acrosomes

中图分类号: 

  • R711.6