关键词: 损伤, 人工骺板, 组织工程, 方法, 软骨细胞

Abstract: Objective To investigate the possibility of constructing artificial epiphyseal plate using combined scaffold. Methods The jelly-like cartilaginous tissue was constructed by mixing epiphyseal plate chondrocyte, 0.3% collagen Ⅰ, 1 000 U·mL^-1 thrombin and 20 g·L^-1 cryodesiccant human fibrinogen. Paraffin sect ion, HE stain, toluidine blue stain and immunohistochemistry were performed regularly. Results Chondrocyte culture used collagen-fibrous protein combined scaffold was baby pink jelly-like, and the cells distributed uniformly. After 3 days, suspended matter took on milkiness, transmittancy depressed. It contracted to 7 mm while hardness increasing. By the method of HE staining, the chondrocyte was round with hyperchromatism of the nuclear, and cartilage matrix was stained powder blue within cartilage cavities in which homologue cell presen ted. The number of cells decreased gradually from surface layer in which the cel l density was biggest to deep zone. The cells were arranged by strata sequence, but didn′t lined as those of epiphyseal plate. There were brown yellow substanc e in cell periphery showed by immunohistochemistry result, which meaned collagen Ⅱ was positive. Conclusion The effect of constructing artificial epiphyseal plate using the combined scaffold is satisfied.

Key words: injuries, artificial epiphyseal plate, tissue engineering, methods, chondrocytes