非特异性角膜炎症反应中PDTC对LPS诱导LFA-1 mRNA表达的影响

doi:国家自然科学基金资助课题(39870313)

非特异性角膜炎症反应中PDTC对LPS诱导LFA-1 mRNA表达的影响

武广恒¹，张家颖¹，杨丽红²，周乐

（1. 吉林大学基础医学院生物化学与分子生物学实验中心，吉林长春130021；2. 天津眼科医院白内障科，天津300020）

收稿日期:2006-06-03 修回日期:1900-01-01 出版日期:2007-07-28 发布日期:2007-07-28
通讯作者: 张家颖

Influence of PDTC on expression of LFA-1 mRNA induced with LPSin mouse non-specific keratitis reaction

WU Guang-heng¹,ZHANG Jia-ying¹,YANG Li-hong²,ZHOU Le_*

1. Experimental Center of Biochemistry and Molecular Biology,School of Basic Medical Sciences,Jilin University,Changchun 130021,China; 2.Department of Cataract, Tianjin Eye Hospital,Tianjin 300020,China)

Received:2006-06-03 Revised:1900-01-01 Online:2007-07-28 Published:2007-07-28
Contact: ZHANG Jia-ying

摘要/Abstract

摘要： 目的：探讨淋巴细胞功能相关抗原-1(LFA-1)的mRNA表达与非特异性角膜炎症反应的相关性及核因子NF-κB抑制剂对LFA-1 mRNA表达的阻断作用。方法：建立BALB/C鼠角膜缝线及联合结膜下药物注射的动物模型，通过逆转录聚合酶链式反应（RT-PCR），对单纯缝线组、缝线联合脂多糖（LPS）组和缝线联合LPS及吡咯啉烷二硫代氨基甲酸盐（PDTC）组术后1、3、7和14 d不同时相点角膜LFA-1 mRNA的转录水平进行检测。结果：术后第1、3、7 d时，角膜缝线联合LPS处理组LFA-1 mRNA的表达明显高于单纯缝线组(P<0.05)；14 d时，角膜缝线联合LPS处理组与单纯缝线组比较差异无显著性（P>0.05）。而1和7 d时缝线联合LPS和PDTC组LFA-1mRNA的表达明显高于缝线联合LPS组；3和14 d时缝线联合LPS和PDTCLFA-1 mRNA的表达明显低于缝线联合LPS组(P<0.05)。结论：联合结膜下注射LPS可在术后诱导LFA-1 mRNA的高水平表达，而核因子NF-κB抑制剂PDTC可抑制角膜LFA-1 mRNA的表达，表明PDTC对LFA-1 mRNA表达具有阻断调控作用。

关键词: NF-kappa B, 角膜炎, 结膜下注射, 吡咯啉烷二硫代氨基甲酸盐, 脂多糖类

Abstract: To analyze the relationship between leukocyte function-associated antigen-1 (LFA-1) mRNA expression and corneal inflammation and discuss the inhibition reaction of nuclear factor-κB（NF-κB）inhibitor on LFA-1 mRNA expression. Methods The corneal suture or combined with subconjunctival injection models were constructed in BALB/C mice.The corneas of each group were excised 1,3,7 and 14 d after operation and LFA-1 mRNA expressions were investigated by reverse transcription polymerase chain reaction(RT-PCR). Results At 1,3 and 7 d after operation, the expressions of corneal LFA-1 mRNA in corneal suture combined with lipopolysaccharide (LPS) (corneal suture+LPs)subconjunctival injection group were higher than those in corneal suture group (P<0.05); at 14 d after operation, there was no significant difference of the expression of corneal LFA-1 mRNA between two groups (P>0.05).At 1 and 7 d after operation, compared with corneal suture+LPS group ,the expression of corneal LFA-1 mRNA increased,but it decreased at 3 and 14 d after operation in the group of corneal suture combined with LPS and pyrrolidine dithiocarbamate (PDTC) (P<0.05). Conclusion A high expression of LFA-1 mRNA can be induced by the corneal stutre combined with LPS subconjunctival injection.The inhibitor of NF-κB (PDTC) can inhibit the expression of corneal LFA-1 mRNA.The expression of LFA-1 mRNA can be blocked by PDTC.

Key words: NF-κB, keratitis, subc onjunctival injection, pyrrolidine dithiocarbamate, lipopolysaccharides

中图分类号:

R722.2

武广恒，张家颖，杨丽红，周乐. 非特异性角膜炎症反应中PDTC对LPS诱导LFA-1 mRNA表达的影响[J]. J4, 2007, 33(4): 612-615.

WU Guang-heng,ZHANG Jia-ying,YANG Li-hong,ZHOU Le. Influence of PDTC on expression of LFA-1 mRNA induced with LPSin mouse non-specific keratitis reaction[J]. J4, 2007, 33(4): 612-615.

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