食管癌患者放射治疗前后外周血淋巴细胞染色体畸变率和微核率的检测及临床意义

doi:卫生部标准处基金项目资助课题（2003）

食管癌患者放射治疗前后外周血淋巴细胞染色体畸变率和微核率的检测及临床意义

于雷¹，王铁君²,王剑锋³，刘丽波¹ ，鞠桂芝¹

1.吉林大学公共卫生学院卫生部放射生物学重点实验室，吉林长春130021；2.吉林大学第二医院放疗科，吉林长春130041；3.吉林大学中日联谊医院放疗科，吉林长春130033

收稿日期:2006-08-17 修回日期:1900-01-01 出版日期:2007-07-28 发布日期:2007-07-28
通讯作者: 刘丽波

Detection of chromosome aberration rate and micronucleus rate in patients with esophageal carcinoma before and after radiotherapy 　and clinical significances

YU Lei¹,WANG Tie-jun²,WANG Jian-feng³,LIU Li-bo¹,JU Gui-zhi¹

1.MH Radiobiology Research Unit,School of Public Health,Jilin University,Changchun 130021,China; 2.Department of Radiotherapy,Second Hospital,Jilin University,Changchun 130041,China; 3.Department of Radiotherapy,China-Japan Union Hospital,Jilin University,Changchun 130033,China

Received:2006-08-17 Revised:1900-01-01 Online:2007-07-28 Published:2007-07-28
Contact: LIU Li-bo

摘要/Abstract

摘要： 目的：探讨食管癌放疗前后外周血淋巴细胞染色体畸变率和微核率的变化。方法：将符合入组条件的食管癌患者21例随机分成两组，Ⅰ组（n=10）采用普通放疗（照射野的形状为规则矩形野的放疗方法）；Ⅱ组（n=11）采用适形放疗（照射野的形状与靶区形状一致的放疗方法），1.8～2.0 Gy/次，1次/日，5次/周，总剂量60～70 Gy。于放射治疗前后采外周静脉血，分析染色体畸变率和微核率。结果：普通放疗后与放疗前比较染色体畸变率和微核率均增高（P<0.05），适形放疗后染色体畸变率和微核率比放疗前亦有提高，但差异无显著性（P>0.05）。结论：与普通放疗相比，适形放疗可减轻细胞遗传学的损伤；检测外周血淋巴细胞染色体畸变率和微核率可作为评价放疗引起食管癌患者细胞遗传学损伤的简单方法。

关键词: 放射疗法, 染色体畸变, 微核

Abstract: Abstract:Objective To study the change of chromosome aberration rate and micronucleus rate in patients with esophageal carcinoma before and after radiotherapy.Methods Twenty one patients with esophageal carcinoma who were fit for conditions were divided into two groups randomly.GroupⅠ（n=10）,patients received conventional radiotherapy（radiation field was regular rectangular shape）；groupⅡ(n=11),patients received conformal radiotherapy（radiation field was same to the shape of target volume）,1.8-2.0 Gy,one time a day,5 times a week,the total dose was 60-70 Gy.The peripheral blood was obtained before and after radiotherapy,chromosome aberration rate and micronucleus rate were detected.Results The chromosome aberration rate and micronucleus rate were increased after conventional radiotherapy than before （P<0.05），they were also increased after conformal radiotherapy than before,but there was no statistics difference（P>0.05）.Conclusion Compared with the conventional radiotherapy,the conformal radiotherapy can decrease cytogenetics damage,the detection of chromosome aberration rate and micronucleus rate can be regarded as a simple method to evaluate cytogenetics damage in esophageal carcinoma patients induced by radiotherapy.

Key words: radiotherapy, chromosome aberrations, 　micronuclei

中图分类号:

R735.1

于雷，王铁君,王剑锋，刘丽波，鞠桂芝. 食管癌患者放射治疗前后外周血淋巴细胞染色体畸变率和微核率的检测及临床意义[J]. J4, 2007, 33(4): 737-740.

YU Lei,WANG Tie-jun,WANG Jian-feng,LIU Li-bo,JU Gui-zhi. Detection of chromosome aberration rate and micronucleus rate in patients with esophageal carcinoma before and after radiotherapy 　and clinical significances[J]. J4, 2007, 33(4): 737-740.

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