单独或联合应用rhG-CSF与bFGF对大鼠急性心肌梗塞的治疗作用

doi:吉林省科技厅基金项目资助课题（990581－3

单独或联合应用rhG-CSF与bFGF对大鼠急性心肌梗塞的治疗作用

胡洋^1,2，陈冬梅¹，潘红丽¹，尉海涛¹，刘全¹

（1.吉林大学第一医院心内科，吉林长春130021；2.吉林省长春市中心医院ICU科，吉林长春 130051）

收稿日期:2007-08-25 修回日期:1900-01-01 出版日期:2008-01-28 发布日期:2008-01-28
通讯作者: 刘全

Effects of using rhG-CSF and bFGF alone or combined on acute myocardial infarction in rats

HU Yang^1,2,CHEN Dong-mei¹,PAN Hong-li¹,WEI Hai-tao¹,LIU Quan¹

(1.Department of Cardiology,First Hospital,Jilin University,Changchun 130021,China;2.Department of ICU,Central Hospital of Changchun of Jilin Province,Changchun 130051,China)

Received:2007-08-25 Revised:1900-01-01 Online:2008-01-28 Published:2008-01-28
Contact: LIU Quan

摘要/Abstract

摘要： 目的：研究重组人粒细胞集落刺激因子（rhG-CSF）与碱性成纤维细胞生长因子（bFGF）单独或联合腹腔注射对大鼠急性心肌梗死（AMI）的治疗作用。方法：结扎大鼠冠状动脉前降支制作AMI模型。成活大鼠随机分为4组：心梗对照组（MI）、rhG-CSF组（G）、bFGF组（B）和联合治疗组（GB），造模后24 h开始依次分别腹腔注射生理盐水、rhG-CSF、bFGF和rhG-CSF+bFGF。另设假手术组（S），只开胸不造模，不给药。分别计数注射前和注射后1周大鼠外周血白细胞（WBC）和单个核细胞（MNC）百分比，心梗后1周免疫组化染色观察CD34⁺细胞表达，HE染色计数新生毛细血管，心梗后4周HE染色观察心肌病理改变，计数毛细血管，测量心功能和梗死面积。结果：G、B、GB组与MI组比较，及GB组与G组或B组相比，外周血WBC计数、MNC百分比、梗死区周围CD34⁺细胞计数、毛细血管密度升高（P<0.01），梗死面积减小（P<0.01），+dp/dt_max、－dp/dt_max和LVSP增高（P<0.01），LVDP降低（P<0.01）。结论：腹腔注射rhG-CSF可以动员大量心肌样细胞；腹腔注射rhG-CSF或bFGF都可以促进血管侧支循环建立,增加梗死区毛细血管数量，两者都可缩小梗死面积，改善心脏的收缩功能；联合应用较单独应用效果更佳。

关键词: 粒细胞集落刺激因子, 碱性成纤维细胞生长因子

Abstract: To study the effects of injecting recombinant human granulocyte colony stimulating factor (rhG-CSF) and basic fibroblast growth factor (bFGF) alone or combined on actue myocardial infarction(AMI).Methods AMI models were induced by ligation of the left anterior descending artery.The survived rats were divided into four groups randomly：AMI group (MI),rhG-CSF group (G),bFGF group (B),combined group (GB).Respectively,saline,rhG-CSF,bFGF,and rhG-CSF plus bFGF were injected intraperitoneally 24 h after AMI.Also,sham-operated group (S) was established with only chest-opeaned,without ligation,and no drugs intervention. The white blood cells (WBC) and mononuclear cells (MNC) proportion in peripheral blood were counted 1 week before and 1 week after the intervention,and the number of CD34⁺ cells was observed with immunohistochemical staining 1 week after AMI in order to compare the situation of mobilization in peripheral blood;the capillary density was evaluated by HE staining both 1 and 4 weeks after AMI;their cardiac fuction was determined in vivo,the infarction size in each group was calculated,and the pathological changes in rat myocardium were observed by HE s taining 4 weeks after AMI.Results Compared with MI group,the number of WBC and MNC% in peripheral blood 1 week after AMI in G,B and GB groups were higher（P<0.01）; LVSP,+dp/dt_max and －dp/dt_max in vivo in G,B and GB groups increased further（P<0.01）and LVDP decreased lower（P<0.01）. Conclusion Injecting rhG-CSF intraperitoneally can mobilize bone marrow stem cells into peripheral blood in infarct rats.The mobilized stem cells may enter into the infarct area and induce the regeneration of cardiac-like cells and capillary endothelial cells.Injecting rhG-CSF or bFGF intraperitoneally may also promote the regeneration of capillary in infarct area.Both of them can improve the cardiac function significantly.rhG-CSF combined with bFGF would be better than use alone.

Key words: granulocyte colony stimulating factor, basic fibroblast growth factor

中图分类号:

R542.22

胡洋，陈冬梅，潘红丽，尉海涛，刘全. 单独或联合应用rhG-CSF与bFGF对大鼠急性心肌梗塞的治疗作用[J]. J4, 2008, 34(1): 73-77.

HU Yang,CHEN Dong-mei,PAN Hong-li,WEI Hai-tao,LIU Quan. Effects of using rhG-CSF and bFGF alone or combined on acute myocardial infarction in rats[J]. J4, 2008, 34(1): 73-77.

[1]	刘文斌, 徐岩, 李智萌, 周佳, 黄晓巍. 参红补血颗粒对血虚证小鼠肾脏组织中EPOmRNA和骨髓组织中GM-CSFmRNA表达水平的影响[J]. 吉林大学学报(医学版), 2017, 43(03): 538-542.
[2]	高建宏, 高金荣, 杨雅琴, 左志洪, 蒙晓玲, 贾俊茹. 聚焦超声治疗前后外阴白色病变组织中VEGF、bFGF和CD34表达的检测及其意义[J]. J4, 2011, 37(1): 130-133.
[3]	林玲, 林晓, 王娅兰. 大肠癌组织中PARG与血管生成相关因子的关系[J]. J4, 2010, 36(3): 439-442.
[4]	王晓杰, 李晶, 田海山, 马吉胜, 周鑫, 刘孝菊, 王艳芳, 姚娜, 李校堃. 重组人碱性成纤维细胞生长因子促进急性心肌梗死大鼠缺血心肌血管再生[J]. J4, 2009, 35(6): 979-979-982.
[5]	程敏, 刘晓红, 程琳, 冯志远. 碱性成纤维细胞生长因子在牙发育各阶段中的表达及意义[J]. J4, 2009, 35(5): 829-831.
[6]	赵月萍, 王志伟, 李校堃, 姚成灿. bFGF/胶原缓释海绵在根尖诱导成形术中的应用[J]. J4, 2006, 32(3): 498-500.
[7]	杨芳，李薇，苑小星，王冠军. 三氧化二砷对小鼠骨髓造血微环境的影响[J]. J4, 2006, 32(3): 400-403.