I粒子植入对大鼠脑内胶质瘤细胞增殖的抑制作用

doi:吉林省科技厅资助课题（200505229）

I粒子植入对大鼠脑内胶质瘤细胞增殖的抑制作用

黄海燕,洪新雨,陈阳,罗毅男^*,李柏

吉林大学第一医院神经外科,吉林长春130021

收稿日期:2005-09-12 修回日期:1900-01-01 出版日期:2006-09-28 发布日期:2006-09-28
通讯作者: 罗毅男

Inhibitory effect of particle ¹²⁵I on proliferationof intracranial glioma cells in rats

HUANG Hai-yan,HONG Xin-yu,CHEN Yang,LUO Yi-nan^*,LI Bai

Department of Neurosugery, First Hospital,Jilin University,Changchun 130021,China

Received:2005-09-12 Revised:1900-01-01 Online:2006-09-28 Published:2006-09-28
Contact: LUO Yi-nan

摘要/Abstract

摘要： 目的：探讨 ¹²⁵I治疗人脑胶质瘤的可行性及其机制。方法：体外培养人胶质瘤细胞（SHG-44），采用MTT法检测 ¹²⁵I粒子对 SHG-44细胞增殖抑制的影响;采用立体定向的方法建立大鼠脑内人胶质瘤模型，接种1周后经MRI检测脑内形成实体瘤。将建模成功大鼠随机分成实验组(n=30)与对照组(n=10)。实验组大鼠在当天肿瘤区域植入 ¹²⁵I粒子1粒，植入粒子1周后复查MRI，2周后处死大鼠，取对照组、实验组肿瘤周边组织及肿瘤组织做增殖细胞核抗原（PCNA）免疫组化检测。结果：MTT法检测SHG-44细胞经¹²⁵I粒子照射后，增殖受到明显抑制，接种1周后脑内形成实体瘤；¹²⁵I可抑制肿瘤细胞生长，延长荷瘤鼠生存期，PCNA基因表达受抑制。结论：¹²⁵I抑制体外培养的SHG-44细胞生长和颅内接种的脑胶质瘤生长的作用机制可能与抑制PCNA基因表达有关。

关键词: 立体定位技术, ^125<, sup>I, 增殖细胞核抗原, 基因

Abstract: Objective To investigate the possibility and mechanism of ¹²⁵I in treatment of glioma. Methods SHG-44 glioma cells were cultivated in vitro, the inhibitory effect of¹²⁵I on SHG-44 cell proliferation was determined by MTT method. The stereotactic method was used to establish the rat intracranial glioma model. The MRI was performed at 1st week after implantation and ²⁵I was implanted in the glioma area, the MRI was performed to measure the diameter of tumor 2 weeks after implantation. The rats were killed after 2 weeks ,PCNA gene experession was determined with immunohistological method both in control and experiment group.Results one week after implantation the glioma grew,the results of MTT method showed the growth of the SHG-44 was inhibited, ¹²⁵I inhibited the expression of PCNA gene and enlonged the rat survival period. Conclusion ¹²⁵I can inhibit the growth of glioma ,the mechanism may be concerned with its inhibitory effect on PCNA gene expression.

Key words: stereotaxic technique, ^125<, sup>Ⅰ, proliferating cell nuclear antigen, gene

中图分类号:

R817.8

黄海燕,洪新雨,陈阳,罗毅男,李柏. I粒子植入对大鼠脑内胶质瘤细胞增殖的抑制作用[J]. J4, 2006, 32(5): 804-807.

HUANG Hai-yan,HONG Xin-yu,CHEN Yang,LUO Yi-nan,LI Bai. Inhibitory effect of particle ¹²⁵I on proliferationof intracranial glioma cells in rats[J]. J4, 2006, 32(5): 804-807.

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