关键词: 骨, 蛋白质组学, 光谱分析, 质量

Abstract: Objective To isolate and identify the proteins responsible for aggressive character of giant cell tumor of bone (GCTB) with proteomic techniques. Methods 5 cases of aggressive GCTB and 4 cases of benign GCTB w ere selected as experimental and control groups, respectively, the differential protein expression were determined by isoelectric focusing / SDS acrylamide gel two-dimensional electrophoresis and Coomassie brilliant blue staining, then the differentially expressed proteins were identified by mass spectrographic analysis. Results(506±23) protein stains were found in experimental group, (468±28) protein stains were found in control group, there was significant difference between the two groups on two-dimensional electrophoresis (P<0.05 ), up-regulation of Annexin A1 and 14-3-3 protein ε, down- regulation of Peroxiredoxin 1 were found in experimental group. Conclusion The down-regulation of Peroxiredoxin 1 may be responsible for the aggression of GCTB, the up-regulation of Annexin A1 and 14-3-3 protein ε may be due to the result of reaction of organism, these three proteins may be selected as tumor markers of the aggressive GCTB.

Key words: proteomics, spectrum analysis, mass