关键词: H-22肝癌细胞, 免疫功能, 预防作用

Abstract: Abstract:Objective To explore the preventive effects of egg-milk with metallothionein (MT) on the H-22 hepatocellular carcinoma in BALB/c mice. Methods Seventy female BALB/c mice were divided into four groups at random: normal control (n=10), tumor control (n=20), low dose egg-milk with MT group (n=20) and high dose egg-milk with MT group (n=20). The mice were injected with H-22 cells subcutaneously to build hepatocellular carcinoma model after they were administered with egg-milk with MT for two weeks. The developing time of tumor and tumor volume were detected. The mice were killed after one month and immune indices were measured with their spleens. The ability of lymphocyte proliferation was measured with MTT method, the percentages of CD4⁺, CD8⁺ and CD25⁺ T cells and apoptotic percentage of lymphocytes were measured with flow cytometry, the killing activities of CTL and NK cells were detected with ³H-TdR method. Results As compared with tumor group, the tumor developing speed in the mice administered with egg-milk with MT decreased significantly (P<0.05), the lymphocyte proliferation rate increased significantly (P<0.01), the positive percentages of CD4⁺, CD8⁺ T cells and the radio of CD4⁺/CD8⁺ T cells increased significantly (P<0.05 or P<0.01), the positive percentage of CD25⁺ T cells and their apoptotic percentages decreased significantly (P<0.05 or P<0.01), the toxicities of NK and CTL cells increased significantly (P<0.05 or P<0.01). Conclusion Egg-milk with MT could enhance the immune function in the mice with hepatocyte tumor and inhibit the tumor developing speed obviously.

Key words: H-22 hepatocyte tumor, immune function, preventive effects