关键词: 磷酸三钙, 骨髓基质细胞, 组织工程, 生物相 容性材料

Abstract: Objective To study the effect of chitosan／tricalcium phosphate（CTCP）on the attachment and proliferation of bone marrow stromal cells（BMSCs）cultivated in vitro and explore their biocompatibilities. Methods The CTCP was prepared by freeze-drying. BMSCs were cultivated in vitro, collected and seeded onto the surface of CTCP.Cell attachment and proliferation were obesrved using inverted light microscope and scanning electron microscopy（SEM）. The cell proliferation was tracked by MTT method 2，4，6，8 d after seeding. Results BMSCs were fibroblast-like 2 d after seeding. The mean number of cells was (360±20) and (76±18)， respectively, in the experimental and control group per 100 eyesight field, the difference was significant (P＜0.01）. MTT showed that the cells kept on proliferating in both groups, in favor of the experimental group. There was significant difference of the absorptio n value between two groups 2，4，6 and 8 d after seeding,respectively（P＜0.01）. SEM showed the porous structure of the CTCP and the seeded BMSCs were well attached to the CTCP with cytoplasmic extensions and lamellipodia. Conclusion CTCP could enhance the proliferation of BMSCs in vitro.The BMSCs have biocompatibility with CTCP,and CTCP could be used as vector for BMSCs in tissue engineering.

Key words: tricalcium phosphate, bone marrow stromal cells, tissue engineering, biocompatible materials