小鼠非清髓性半相合骨髓移植造血的重建

doi:全军医药卫生科研基金十五重点项目资助课题

小鼠非清髓性半相合骨髓移植造血的重建

王春燕¹,2，郭坤元^1*，姜振宇¹,2，吴岚晓¹

1.第一军医大学珠江医院血液科，广东广州510282；2.吉林大学第一医院血液肿瘤科，吉林长春130021

收稿日期:2004-03-17 修回日期:1900-01-01 出版日期:2004-11-28 发布日期:2004-11-28
通讯作者: 郭坤元

Hematopoietic reconstitution of nonmyeloablative MHChaplotype-mismatched BMT in mice

WANG Chun-yan^1,2, GUO Kun-yuan^1*, JIANG Zhen-yu¹,2, WU Lan-xiao¹

1. Department of Hematology, Zhujiang Hospital，First Military University, Guangzhou 510282，China；2. Department of Hematology and Oncology, First Hospital, Jilin University, Changchun 130021, China

Received:2004-03-17 Revised:1900-01-01 Online:2004-11-28 Published:2004-11-28
Contact: GUO Kun-yuan

摘要/Abstract

摘要： 目的：探讨在非清髓性预处理条件下，纯化的供鼠NK细胞对MHC半相合小鼠骨髓移植（BMT）造血重建的影响。方法：以CB6F₁^H-2d/b（BALB/c^H-2d×C57BL／6^H-2b）小鼠为受者，C57BL／6^H-2b小鼠为供者。60只受者小鼠根据不同照射剂量及不同NK细胞输注量随机分为10组，每组6只，经9.0、7.0、6.0 Gy 3个剂量⁶⁰Co照射。经9.0 Gy照射后分为单纯照射组（Ⅰ）、半相合BMT组（Ⅱ）。经7.0 Gy照射后分为单纯照射组（Ⅲ）、半相合BMT组（Ⅳ）、NK细胞移植1组（Ⅴ）和NK细胞移植2组（Ⅵ）。经6.0 Gy照射后分为单纯照射组（Ⅶ）、半相合BMT组（Ⅷ）、NK细胞移植1组（Ⅸ）和NK细胞移植2组（X）。在照射后4 h移植供鼠骨髓细胞，其中NK细胞移植1组（Ⅴ、Ⅸ）每只受鼠移植前输注纯化供鼠NK细胞1×106，NK细胞移植2组（Ⅵ、X）每只受鼠移植前输注供鼠NK细胞5×10⁵。以血常规、体重、生存期等变化为指标，观察60 d。结果：①生存期：Ⅰ组为（5.83±0.98）d，其他组小鼠生存期均大于60 d。②移植后第10天时白细胞及血小板计数：Ⅴ、Ⅵ组WBC和PLT分别为（1.05±0.30）×10⁹•L^-1、（53.50±12.11）×10⁹•L^-1及（0.95±0.26）×10⁹•L^-1、（47.17±11.82）×10⁹•L^-1，明显高于Ⅲ、Ⅳ组（P<0.01）；Ⅸ、X组WBC和PLT分别为（0.98±0.34）×10⁹•L^-1、（50.67±12.58）×10⁹•L^-1及（0.88±0.29）×10⁹•L^-1、（45.33±14.36）×10⁹•L^-1，明显高于Ⅶ、Ⅷ组（P<0.05）。结论：MHC半相合BMT小鼠移植前输注纯化的供鼠NK细胞促进其造血重建。

关键词: 天然, 骨髓移植, 非清髓性, 造血重建

Abstract: Objective To study the relationship between donor-derived NK cells and hematopoietic reconstitution after nonmyeloablative MHC haplotype-mismatched BMT in mice. Methods The murine model of MHC haplotype-mismatched BMT was established by using (BALB/c^H-2d×C57BL／6^H-2b) CB6F₁^H-2d/b mouse as the recipient, and C57BL／6^H-2b mouse as the donor. 60 recipient mice were divided into 10 groups after irradiation (TBI ⁶⁰Co 9.0,7.0， and 6.0 Gy)：9.0 Gy-irradiation groups including simple-irradiation group (Ⅰ), MHC haplotype-mismatched BMT group(Ⅱ); 7.0 Gy-irradiation groups including simple-irradiation group (Ⅲ), MHC haplotype-mismatched BMT group (Ⅳ), the first of NK cells-transplantation group (Ⅴ), the second of NK cells-transplantation group (Ⅵ), 6.0 Gy-irradiation groups including simple-irradiation group (Ⅶ), MHC haplotype-mismatched BMT group (Ⅷ), the first of NK cells-transplantation group (Ⅸ), the second of NK cells-transplantation group (X). The recipients were infused into the bone marrow cells of donors when they were irradiated after 4 hours. The recipients, the first of NK cells-transplantation groups, were infused into NK cells of 1×10⁶/one; The recipients, the second of NK cells-transplantation groups, were infused into NK cells of 5×10⁵/one. The effects were assessed by the count of WBC and PLT, survival time, body weight in the recipients. Results ①Life span: survival time was (5.83±0.98) days in group Ⅰ, beyond 60 days in other groups. ②Leukocyte and platelet counts after 10 days: leukocyte and platelet counts were (1.05±0.30)×10⁹• L^-1,（53.50±12.11）×10⁹•L^-1 and （0.95±0.26）×10⁹•L^-1,（47.17±11.82）×10⁹•L^-1 in group Ⅴ and group Ⅵ, which were much higher than those in group Ⅲ and group Ⅳ (P<0.01); In the cases of group Ⅸ and group X, leukocyte and platelet counts were （0.98±0.34）×10⁹•L^-1,（50.67±12.58）×10⁹•L^-1;（0.88±0.29）×10⁹•L^-1,（45.33±14.36）×10⁹•L^-1, which were much higher than those in group Ⅶ and group Ⅷ (P<0.05). Conclusion Donor-derived NK cells can promote hematopoietic reconstitution after nonmyeloablative MHC haplotype-mismatched BMT in mice.

Key words: natural, bone marrow transplantation, nonmyeloablative, hematopoietic reconstitution

中图分类号:

R457.7

王春燕,2，郭坤元，姜振宇,2，吴岚晓. 小鼠非清髓性半相合骨髓移植造血的重建[J]. J4, 2004, 30(6): 908-911.

WANG Chun-yan, GUO Kun-yuan, JIANG Zhen-yu,2, WU Lan-xiao. Hematopoietic reconstitution of nonmyeloablative MHChaplotype-mismatched BMT in mice[J]. J4, 2004, 30(6): 908-911.

[1]	张丽鹏, 荣春书, 谷一鸣, 傅耀文, 高宝山. 肾-骨髓联合移植诱导免疫耐受患者外周血B淋巴细胞重构的动态特点及其意义[J]. 吉林大学学报(医学版), 2018, 44(02): 332-338.
[2]	毕云枫,宋凤瑞,刘志强. 天然酪氨酸酶抑制剂的种类及其对酪氨酸酶抑制作用的研究进展[J]. 吉林大学学报(医学版), 2014, 40(02): 454-459.
[3]	贺军\|周丽婷\|李娜\|刘特\|郑东春\|曲笑锋\|黄鉴. ICP-MS法测定长白山天然矿泉水中18种微量元素的含量[J]. J4, 2012, 38(6): 1223-1226.
[4]	付彤,李嗣杰,宋东，范志民. 艾易舒注射液在乳腺癌术后辅助治疗中的应用及效果评价[J]. J4, 2007, 33(4): 744-746.
[5]	郭坤元，梅家转，姜振宇，姚开泰. NK细胞与K562细胞之间相互免疫编辑作用及其对NK细胞杀伤活性的影响[J]. J4, 2007, 33(4): 630-633.
[6]	王春燕，谭获，郭坤元. 异基因性NK细胞在小鼠体内抗白血病作用[J]. J4, 2007, 33(4): 658-661.
[7]	马岩，张锐，于小风，曲绍春，徐华丽，睢大员. 黄蘑多糖提取物的抗肝癌作用及其机制[J]. J4, 2005, 31(6): 886-889.
[8]	马岩，马颖哲，张家颖，李晓梅. 黄蘑多糖Fb对小鼠免疫功能的调节作用[J]. J4, 2005, 31(5): 692-695.
[9]	王春燕，郭坤元，姜振宇,吴秉毅，吴岚晓. 供者NK细胞诱导小鼠MHC半相合骨髓移植的免疫耐受[J]. J4, 2004, 30(2): 188-190.