辛伐他汀对病毒性心肌炎小鼠心肌细胞L-型钙通道mRNA表达的影响

doi:吉林省科技厅科技发展计划项目资助课题（20

辛伐他汀对病毒性心肌炎小鼠心肌细胞L-型钙通道mRNA表达的影响

窦忠霞¹，王举²，魏征人³，孙景辉¹

1.吉林大学第一医院小儿心血管科，吉林长春 130021; 2.吉林大学第一医院结直肠肛门外科，吉林长春 130021; 3.吉林大学基础医学院药理学教研室，吉林长春 130021

收稿日期:2007-04-08 修回日期:1900-01-01 出版日期:2009-01-28 发布日期:2009-01-28
通讯作者: 孙景辉

Effect of simvastatin on gene expression of L-type calcium channels in mouse myocardium with myocarditis caused by coxsackievirus B3

DOU Zhong-xia¹，WANG Ju²，WEI Zheng-ren³，SUN Jing-hui¹

1. Department of Pediatric,First Hospital,Jilin University,Changchun 130021,China；2. Department of Colonrectal and Anus Surgery,First Hospital,Jilin University,Changchun 130021,China；3. Department of Pharmacology，School of Basic Medical Sciences,Jilin University,Changchun 130021,China

Received:2007-04-08 Revised:1900-01-01 Online:2009-01-28 Published:2009-01-28
Contact: SUN Jing-hui

摘要/Abstract

摘要： 目的:研究辛伐他汀对柯萨奇B3病毒（CVB3）感染BALB/c小鼠后心肌细胞 L-型钙通道(LCCs)mRNA表达的影响，探讨辛伐他汀对病毒性心肌炎的治疗作用。方法：应用 CVB3 感染BALB/c小鼠制作病毒性心肌炎模型，辛伐他汀混悬液灌胃，按辛伐他汀剂量分为10 mg•kg^-1 组、30 mg•kg^-1 组及90 mg•kg^-1 组，并设正常对照组及病毒感染对照组，行心肌病理观察，采用半定量逆转录-聚合酶链式反应(RT-PCR)检测正常对照组、病毒感染组及辛伐他汀组心肌细胞LCCs　α₁亚单位mRNA 表达量。结果:病毒感染组小鼠心肌组织有局灶性或弥漫性以单核细胞为主的炎性细胞浸润，重者可见大片状心肌细胞坏死；辛伐他汀组心肌组织炎症细胞浸润及坏死灶明显减轻；正常对照组和病毒模型组心肌LCCs α₁亚单位的mRNA表达量分别为0.06±0.01和1.37±0.32，二者比较差异有显著性（P<0.05）；辛伐他汀10 mg•kg^-1、30 mg•kg^-1 及90 mg•kg^-1 组LCCs α₁ 亚单位的mRNA表达量分别为1.14±0.22、0.17±0.04和0.11±0.02，与病毒模型组比较差异有显著性（P<0.05）,其中以中、高剂量组下降最明显。结论:辛伐他汀可减轻病毒性心肌炎的病理损害，并以剂量依赖的方式抑制心肌细胞LCCs α₁ 亚单位的mRNA表达,发挥心肌保护功能，对病毒性心肌炎有一定的治疗作用。

关键词: 心肌炎, L-型钙通道

Abstract: Abstract:Objective To investigate the effect of simvastatin on gene expression of L-type calcium channels(LCCs) of myocardial cells in BALB/c mice infected by coxsackievirus B3(CVB3 ) so as to study the therapeutic effect of simvastatin on viral myocarditis.Methods Sixty male BALB/c mice were divided into five groups randomly(n=12).Mice in viral control group and three groups of oral administration of simvastatin(10,30 and 90 mg•kg^-1)were inoculated intrapritoneally with 0.2 mL of CVB3(Nancy strain).Mice in normal control group were inoculated intrapritoneally with 0.2 mL Eagle’〖KG-*3〗s solution.The heart samples of all the mice were obtained for hispathological study and detection of myocardial LCCs alpha1 subunits mRNA expression by semi-quantitative reverse transcription- polymerase chain reaction (RT-PCR).Results In viral control group,the mononuclear inflamematory infiltrate was focal or diffuse in myocardium of mice,severe hearts revealed a large area of myocardial necrosis.The degree of inflammatory cell infiltrate and area of necrosis were significantly less in simvastatin groups as compared with viral control group.The myocardial LCCs alpha1 subunits mRNA expression by semi -quantitaive RT-PCR in normal control group was much lower than that in viral control group (0.06±0.01 vs 1.37±〖JP〗0.32,P<0.05); the expression levels of myocardial LCCs alpha1 subunits mRNA in simvastatin(10,30 and 90 mg•kg^-1)  groups were 1.14±0.22,0.17±0.04 and 0.11±0.02,respectively,and they were lower than that in viral control group(Ｐ<0.05); In simvastatin(30 and 90 mg•kg^-1) groups,the myocardial LCCs alpha1 subunits mRNA expressions were significantly decreased(P<0.05). Conclusion Simvastatin could reduce the grade of myocardial damage and block virus-induced LCCs alpha1 subunit gene expression in a dose-dependent manner,which might exert a protective effect in myocarditis mice infected by CVB3.

Key words: myocarditis, L-type calcium channel

中图分类号:

R331.31

窦忠霞，王举，魏征人，孙景辉. 辛伐他汀对病毒性心肌炎小鼠心肌细胞L-型钙通道mRNA表达的影响[J]. J4, 2009, 35(1): 78-81.

DOU Zhong-xia，WANG Ju，WEI Zheng-ren，SUN Jing-hui. Effect of simvastatin on gene expression of L-type calcium channels in mouse myocardium with myocarditis caused by coxsackievirus B3[J]. J4, 2009, 35(1): 78-81.

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