低剂量辐射对人骨髓间充质干细胞造血生长因子表达量的影响

doi:国家自然科学基金资助课题（30370439）；长

低剂量辐射对人骨髓间充质干细胞造血生长因子表达量的影响

杨岩¹，王娟²，王冠军¹，朱婧妍^*

1.吉林大学第一医院血液科，吉林长春130021；2.吉林省人民医院肿瘤科，吉林长春130021

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-09-28 发布日期:2008-09-28
通讯作者: 王冠军

Effect of low dose radiation on expression of hematopoietic growth factors secreted by human mesenchymal stem cells from bone marrow

YANG Yan¹，WANG Juan², WANG Guan-Jun¹，ZHU 　Jing-yan^*

1.Department of Hematology，First Hospitol， Jilin University，Changchun 130021，China；2.Department of Oncology，Pepeol’[KG-*3]s Hospital of Jilin ProvinceChangchun 130021，China

Received:1900-01-01 Revised:1900-01-01 Online:2008-09-28 Published:2008-09-28
Contact: WANG Guan-Jun

摘要/Abstract

摘要： 目的：探讨低剂量辐射（LDR）对人骨髓间充质干细胞（BM-MSC）的兴奋性效应及其分子机制。方法：应用体外传代培养的P4和P5 BM-MSC，采用X射线照射，分为50、75和100 mGy 3个照射剂量组，剂量率为12.5 mGy•min^-1，每组细胞设对应假照组，采用ELISA方法检测LDR后BM-MSC细胞因子表达量的变化。结果：与同一时间假照组比较，50、75和100 mGy X射线照射人BM-MSC后，在培养24和48 h时干细胞因子（SCF）分泌量均有升高趋势，仅75 mGy照射后48 h时SCF分泌量明显升高，与同一时间假照组比较差异有显著性（P<0.05）； 50和75 mGy照射组在24和48 h,100 mGy照射组在24 h时IL-6分泌量明显升高，与同一时间假照组比较差异有显著性（P<0.05）；50、75和100 mGy照射BM-MSC后，与同一时间假照组比较，除50 mGy 照射后 72 h外，M-SCF分泌量在照射后在24、48和72 h均明显升高（P<0.05），以75 mGy照射后72 h升高最明显。结论： LDR对BM-MSC有兴奋效应，表现为细胞生长加速，同时在一定时间内可以使造血生长因子表达量增多。

关键词: 低剂量辐射, 造血生长因子

Abstract: Abstract:Objective To study the changes of hematopoietic growth factors secreted by human mesenchymal stem cells from bone marrow(BM-MSC) pretreated with low dose radiation （LDR）.Methods The cultured P4 and P5 BM- MSCs were exposed to X rays atthe doses of 50 ，75 and 100 mGy (dose rate 12.5 mGy•min^-1).The changes of levels of stem cell factor (SCF), IL-6, macrophage colony-stimulating factor( M-CSF) secreted by BM-MSCs pretreated with LDR were determined by ELISA method. Results As compared with control group at the same time, the levels of SCF in experimental group had a tendency of increasing after 24 h and 48 h radiation,but only in 75 mGy group the SCF level was obviously increased (P<0.05). The levels of IL-6 in 50 and 75 mGy groups at 24 h and 48 h ,in 100 mGy group at 24 h were obviously increased compared with control group(P<0.05) .The levels of M-CSF in all the groups at 24 h, 48 h and 72 h except for the 50 mGy dose at 72 h were also increased(P<0.05),it increased markedly in 75 mGy dose group at 72 h.Conclusion LDR has hormesis effect on BM-MSCs .After LDR,the BM-MSCs grow faster and in a certain phase the expression levels of hematopoietic growth factors are increased.

Key words: low dose radiation, hematopoietic growth factors

中图分类号:

R817.1

杨岩，王娟，王冠军，朱婧妍. 低剂量辐射对人骨髓间充质干细胞造血生长因子表达量的影响[J]. J4, 2008, 34(5): 737-740.

YANG Yan，WANG Juan, WANG Guan-Jun，ZHU 　Jing-yan. Effect of low dose radiation on expression of hematopoietic growth factors secreted by human mesenchymal stem cells from bone marrow[J]. J4, 2008, 34(5): 737-740.

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