甲醛对雄性小鼠的生殖遗传毒性

doi:吉林省科技厅基金资助课题（2005138）

甲醛对雄性小鼠的生殖遗传毒性

邢沈阳¹, 叶琳²，王南南³

1.吉林大学农学部畜牧兽医学院遗传育种与繁殖系，吉林长春130062；2.吉林大学公共卫生学院环境卫生学教研室，吉林长春130021；3.山东省卫生厅，山东济南 250014

收稿日期:2006-08-21 修回日期:1900-01-01 出版日期:2007-07-28 发布日期:2007-07-28
通讯作者: 叶琳

Toxic effect of formaldehyde on reproduction and heredity in male mice

XING Shen-yang¹,YE Lin²,WANG Nan-nan³

1.Department of Animal Genetics and Breeding,College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130021,China; 2.Department of Environment Hygiene,School of Public Health,Jilin University,Changchun 130021,China; 3.Department of Health of Shandong Province,Jinan 250014,China

Received:2006-08-21 Revised:1900-01-01 Online:2007-07-28 Published:2007-07-28
Contact: YE Lin

摘要/Abstract

摘要： 目的：观察甲醛对雄性小鼠精子畸变、生殖能力和子代胎鼠肝细胞微核率的影响。方法：随机将小鼠分为阴性对照组（NC）、甲醛染毒组和阳性对照组（PC），每组12只，采用静式吸入染毒，染毒组吸入甲醛浓度分别是21 mg•m-3 (1/24LC₅₀)、42 mg•m-3(1/12 LC₅₀)和84 mg•m-3 (1/6 LC₅₀)，每天2 h，每周6 d，共13周。染毒结束后处死小鼠，检测小鼠精子畸形率，采用显性致死试验方法，检测雄性小鼠生殖能力及子代胎鼠肝细胞微核率。结果：各染毒组雄性小鼠精子头畸形率显著高于阴性对照组(P<0.05)，并与甲醛染毒剂量呈明显正相关(r=0.83,P<0.001)。各剂量染毒组孕鼠的胎吸收率较阴性对照组显著升高(P<0.01)，1/6 LC₅₀组的每窝平均活胎数较阴性对照组显著下降(P<0.05)。结论：甲醛可以造成雄性小鼠精子畸形，降低雄性小鼠生殖能力，对雄性小鼠具有一定的生殖遗传毒性。

关键词: 毒性, 生殖力, 显性致死试验, 精子畸变, 微核

Abstract: Abstract:Objective To examine the effects of formaldehyde on the process of sperm development and reproductive capacity in male mice and the micronucleus rate of liver cells from offspring.Methods The mice were randomly divided into negative control group（NC）,formaldehyde exposed group and positive control group (PC).The exposed dose was 21 mg•m-3 (1/24 LC₅₀),42 mg•m-3 (1/12 LC₅₀) and 84 mg•m-3 (1/6 LC₅₀),respectively. 12 male mice were included in each group.Male mice from different formaldehyde exposed groups were inhaled with formaldehyde for 2 h one day,6 d each week,lasted for 13 weeks.The aberration rate of sperm in male mice and the micronucleus rate of liver cells from offspring were observed,and the reproductive capacity of male mice was detected by dominant lethal test.Results The aberration rates of sperm in mice from different formaldehyde exposed groups were significantly higher than that in negative control (P<0.05),and the relationship between the spermatozoa aberration rate and the concentration of inhaled formaldehyde was positive correlation (r=0.83,P<0.001).The rates of fetal absorption in pregnant mice from three different exposed groups were significantly higher than that in negative control (P<0.01),and the number of live offspring from 1/6　LC₅₀ group was lower than that in negative control (P<0.05).Conclusion Formaldehyde can cause the increasing of sperm aberration rate and the decreasing of the reproductive capacity in male mice.The results suggest that formaldehyde can affect the genetics of male mice.

Key words: fertility, dominant lethal test, spermatozoa aberrations, micronuclei

中图分类号:

R394.6

邢沈阳, 叶琳，王南南. 甲醛对雄性小鼠的生殖遗传毒性[J]. J4, 2007, 33(4): 716-718.

XING Shen-yang,YE Lin,WANG Nan-nan. Toxic effect of formaldehyde on reproduction and heredity in male mice[J]. J4, 2007, 33(4): 716-718.

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