关键词: 毒性, 生殖力, 显性致死试验, 精子畸变, 微核

Abstract: Abstract:Objective To examine the effects of formaldehyde on the process of sperm development and reproductive capacity in male mice and the micronucleus rate of liver cells from offspring.Methods The mice were randomly divided into negative control group（NC）,formaldehyde exposed group and positive control group (PC).The exposed dose was 21 mg•m-3 (1/24 LC₅₀),42 mg•m-3 (1/12 LC₅₀) and 84 mg•m-3 (1/6 LC₅₀),respectively. 12 male mice were included in each group.Male mice from different formaldehyde exposed groups were inhaled with formaldehyde for 2 h one day,6 d each week,lasted for 13 weeks.The aberration rate of sperm in male mice and the micronucleus rate of liver cells from offspring were observed,and the reproductive capacity of male mice was detected by dominant lethal test.Results The aberration rates of sperm in mice from different formaldehyde exposed groups were significantly higher than that in negative control (P<0.05),and the relationship between the spermatozoa aberration rate and the concentration of inhaled formaldehyde was positive correlation (r=0.83,P<0.001).The rates of fetal absorption in pregnant mice from three different exposed groups were significantly higher than that in negative control (P<0.01),and the number of live offspring from 1/6　LC₅₀ group was lower than that in negative control (P<0.05).Conclusion Formaldehyde can cause the increasing of sperm aberration rate and the decreasing of the reproductive capacity in male mice.The results suggest that formaldehyde can affect the genetics of male mice.

Key words: fertility, dominant lethal test, spermatozoa aberrations, micronuclei