乳腺癌患者[WT3HX][ST3HX]CYP1A1[WT3HZ][ST3HZ]基因MspⅠ酶切位点多态性的分析

doi:吉林省科技厅基金资助课题 （20020644）

乳腺癌患者[WT3HX][ST3HX]CYP1A1[WT3HZ][ST3HZ]基因MspⅠ酶切位点多态性的分析

白淑芬^1,2，杨立新²，张爱臣¹，冷维春¹

1.吉林大学中日联谊医院妇产科，吉林　长春 130033；2.江苏省南京市第二医院妇产科，江苏　南京 210003

收稿日期:2008-11-11 修回日期:1900-01-01 出版日期:2009-03-28 发布日期:2009-08-14
通讯作者: 张爱臣

Analysis of polymorphism of CYP1A1-MspⅠ gene in patients with breast cancer

BAI Shu-fen^1,2,YANG Li-xin²，ZHANG Ai-chen¹，LENG Wei-chun¹

1.Department of Obstetrics and Gynecolgy，China-Japan Union Hospital，Jilin Unive

Received:2008-11-11 Revised:1900-01-01 Online:2009-03-28 Published:2009-08-14
Contact: ZHANG Ai-chen

摘要/Abstract

摘要： 目的：探讨细胞色素[CYP1A1基因MspⅠ酶切位点多态性与乳腺癌遗传易感性的关系。方法:应用聚合酶链式反应- 限制性片段长度多态性(PCR-RFLP)分析技术，检测160例乳腺癌患者(病例组)和124例同期住院非乳腺癌患者(对照组)， CYP1A1基因MspⅠ酶切位点多态性的3种基因型及等位基因的频数分布。结果：在病例组CYP1A1基因MspⅠ酶切位点基因型频数分布为：TT（45.0％）、TC（46.3％）、CC（8.8％），等位基因频数分布为T（68.1％）、C（31.9％）；在对照组CYP1A1[基因MspⅠ酶切位点基因型的频数分布为TT（61.3％）、TC（35.5％）、CC（3.2％），等位基因频数分布为T（79.0％）、C（21.0％）。各个基因型在两组中所占的比例差异无显著性（P>0.05）;两组T、C等位基因频数比较差异有显著性(P<0.05)。结论： CYP1A1基因MspⅠ酶切位点多态性可能与本地区乳腺癌易感性有关。

关键词: 细胞色素P450

Abstract: Objective To investigate the association between CYP1A1-MspⅠ gene polymorphism and genetic susceptibility of breast cancer.Methods CYP1A1-MspⅠ gene polymorphism in 160 patients with breast cancer and 124 controls were detected by PCR-RFLP.The frequencies of genotype and allele were analyzed.Results The distribution frequencies of genotype of CYP1A1[-MspⅠ site in case group were TT45.0％,TC46.3％,CC8.8％,and the frequencies of allelic gene were T（68.1％） and C（31.9％）. While the distribution frequences of genotype of CYP1A1-MspⅠ site in control group were TT61.3％,TC35.5％,CC3.2％,and the frequences of allelic gene were T79.0％,C21.0％.The ratios of genotypes in each group had no significant difference（P>0.05）；The frequencies of allelic gene of T and C in each group had significant difference（P<0.01）.ConclusionCYP1A1-MspⅠ gene polymorphism may be linked with genetic susceptibility of breast cancer in this area.

Key words: cytochrome P-450　CYP1A1, polymorphism, singlenucleotide, genetic predisposition to disease

中图分类号:

R737.9

白淑芬，杨立新，张爱臣，冷维春. 乳腺癌患者[WT3HX][ST3HX]CYP1A1[WT3HZ][ST3HZ]基因MspⅠ酶切位点多态性的分析[J]. J4, 2009, 35(2): 377-380.

BAI Shu-fen,YANG Li-xin，ZHANG Ai-chen，LENG Wei-chun. Analysis of polymorphism of CYP1A1-MspⅠ gene in patients with breast cancer[J]. J4, 2009, 35(2): 377-380.

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