携带siRNA-Stat3质粒减毒沙门氏菌对肝原位移植瘤的治疗作用

doi:国家科技部国际科技合作重点项目资助课题（

携带siRNA-Stat3质粒减毒沙门氏菌对肝原位移植瘤的治疗作用

田勇¹，张灵¹，汲坤¹，高丽芳¹，孙莹²，刘艳波^1,3 ，徐德启4，赵雪俭¹

(1.吉林大学基础医学院病理生理学教研室，吉林长春 130021;2.吉林大学第一医院电诊科，吉林长春 130021；3.北华大学病理生理学教研室，吉林吉林 132001；4.美国食品药品监督管理局，马里兰州　贝斯达　MD20892 )

收稿日期:2007-04-18 修回日期:1900-01-01 出版日期:2008-01-28 发布日期:2008-01-28
通讯作者: 赵雪俭

Therapeutic effect of attenuated salmonella typhimurium with siRNA-Stat3 plasmid on orthotopic transplantation tumor model of liver in mice

TIAN Yong¹，ZHANG Ling¹,JI Kun¹,GAO Li-fang¹,SUN Ying²,LIU Yan-bo^1,3,XU De-qi⁴,ZHAO Xue-jian¹

angchun 130021，China;2.Department of Electrodiagnosis，First Hospital，Jilin University，Changchun 130021,China;3. Department of　Pathophysiology，Beihua University,Jilin 132001,China;4.Food and Drug Administration,Bethesda　MD20892,US)

Received:2007-04-18 Revised:1900-01-01 Online:2008-01-28 Published:2008-01-28
Contact: ZHAO Xue-jian

摘要/Abstract

摘要： 目的：观察携带有siRNA-Stat3质粒的鼠减毒沙门氏菌对小鼠肝脏原位移植瘤的治疗作用及效果。方法：将siRNA-Stat3质粒经电转化方法转入减毒鼠沙门氏菌中，将肝原位移植瘤小鼠分为mock未治疗组、pGC-Si-Stat3组和pGC-Si-Scramble组。pGC-Si-Stat3组小鼠灌胃转化有siRNA-Stat3质粒减毒沙门氏菌，pGC-Si-Scramble组小鼠灌胃转化有空质粒的减毒沙门氏菌，未治疗组小鼠灌胃PBS，观察pGC-Si-Stat3组Stat3 mRNA和蛋白表达水平。结果：与未治疗组和pGC-Si-Scramble组比较，携带有siRNA-Stat3质粒治疗组肝原位移植瘤体积明显缩小(P<0.05),瘤重减轻(P<0.05)。与其对应的 mRNA和蛋白表达泳道光密度值较未治疗组和pGC-Si-Scramble组明显降低（P<0.01﹚。结论：携带有siRNA-Stat3质粒的鼠减毒沙门氏菌对小鼠肝原位移植瘤的生长具有显著抑制作用。

关键词: 减毒沙门氏菌, 肝原位移植瘤

Abstract: To study the therapeutic effect of attenuated salmonella typhimurium with siRNA-Stat3 plasmid on orthotopic transplantation tumor model of liver in C57BL/6 mice. Methods The siRNA-Stat3 plasmid was transfected into attenuated salmonella typhimurium after that orthotopic transplantation tumor model of liver in mice were infused with attenuated salmonella typhimurium and then the gene expression and function were observed.Results Compared with mock group and pGC-Si-Scramble group,the growth of orthotopic transplantation tumors of liver was inhibited significantly in siRNA-Stat3 plasmid group(P<0.05),the tumor weight was reduced obviously(P<0.05),the expression levels of protein and mRNA were down-regulated (P<0.01).Conclusion The　 attenuated salmonella typhimurium which carrying siRNA-Stat3 plasmid has the inhibitory effect on orthotopic transplantation tumor of liver.

Key words: attenuated salmonella typhimurium, orthotopic transplantation tumors of liver

中图分类号:

R73-354

田勇，张灵，汲坤，高丽芳，孙莹，刘艳波，徐德启4，赵雪俭. 携带siRNA-Stat3质粒减毒沙门氏菌对肝原位移植瘤的治疗作用[J]. J4, 2008, 34(1): 38-41.

TIAN Yong，ZHANG Ling,JI Kun,GAO Li-fang,SUN Ying,LIU Yan-bo,XU De-qi,ZHAO Xue-jian. Therapeutic effect of attenuated salmonella typhimurium with siRNA-Stat3 plasmid on orthotopic transplantation tumor model of liver in mice[J]. J4, 2008, 34(1): 38-41.