丝裂霉素对小鼠淋巴瘤EL-4细胞P21蛋白表达的影响

doi:国家自然科学基金资助课题

丝裂霉素对小鼠淋巴瘤EL-4细胞P21蛋白表达的影响

闫风琴¹，王建秋²，付士波¹, 鞠桂芝^1*

1.吉林大学公共卫生学院卫生部放射生物学重点实验室，吉林长春130021；2.吉林大学再生医学科学研究所生物化学研究室，吉林长春130021

收稿日期:2004-09-24 修回日期:1900-01-01 出版日期:2005-05-28 发布日期:2005-05-28
通讯作者: 鞠桂芝

Effects of MMC on expressions of P21 protein in EL-4 mouse lymphoma cells

YAN Feng-qin¹，WANG Jian-qiu²，FU Shi-bo¹, JU Gui-zhi^1*

1.MH Radiobiology Research Unit, School of Public Health, Jilin University, Changchun 130021, China;2. Department of Biochemistry, Institute of Frontier Medical Sciences, Jilin University, Changchun 130021, China

Received:2004-09-24 Revised:1900-01-01 Online:2005-05-28 Published:2005-05-28
Contact: JU Gui-zhi

摘要/Abstract

摘要： 目的：探讨丝裂霉素（MMC）对小鼠淋巴瘤EL-4细胞P21蛋白表达的影响。方法：采用免疫荧光染色及流式细胞术检测P21蛋白表达的时程和量效改变。结果：时程实验研究证实，给予2 mg•L^-1 MMC处理后，EL-4细胞中P21蛋白表达在2～48 h 明显高于对照组（P<0.05, P<0.01）。量效实验结果证实，给予1、2和4 mg•L^-1 MMC处理后24 h，P21蛋白表达与对照组相比明显增高（P<0.01）。结论：MMC能诱导P21蛋白表达增高，并存在时间和剂量依赖关系。

关键词: 淋巴瘤EL-4细胞, P21蛋白

Abstract: Objective To explore the effects of mitomycin (MMC) on the expressions of P21 protein in EL-4 mouse lymphoma cells. Methods Flow cytometry and immunofluorescence staining were used to measure P21 protein expressions in changes with time and doses. Results In time-course experiments, it was demonstrated that P21 protein levels were markedly increased at 2-48 h after treatment with 2 mg•L^-1 MMC in EL-4 cells compared with control group (P<0.01, P<0.05). In dose-effect experiments, it was showed that P21 protein expressions were increased significantly 24 h after treatment with 1，2，and 4 mg•L^-1 MMC in EL-4 cells compared with control group (P<0.01). Conclusion The P21 protein expression could be increased by MMC in time- and dose-dependent manners.

Key words: EL-4 mouse lymphoma cells, P21 protein

中图分类号:

R-332

闫风琴，王建秋，付士波, 鞠桂芝. 丝裂霉素对小鼠淋巴瘤EL-4细胞P21蛋白表达的影响[J]. J4, 2005, 31(3): 340-342.

YAN Feng-qin，WANG Jian-qiu，FU Shi-bo, JU Gui-zhi. Effects of MMC on expressions of P21 protein in EL-4 mouse lymphoma cells[J]. J4, 2005, 31(3): 340-342.