原发性胰腺癌患者高强度聚焦超声治疗前后外周血CD4+CD25+
调节性T细胞的变化及其意义

吉林大学学报(医学版)

原发性胰腺癌患者高强度聚焦超声治疗前后外周血CD4+CD25+
调节性T细胞的变化及其意义

宋晓燕¹，马宁²，姜振宇²，张彦东²，杜冰洋¹

1. 吉林省吉林市中心医院肿瘤诊治中心二组,吉林吉林 132011；2. 吉林大学第一
医院风湿免疫科,吉林长春 130021

收稿日期:2013-01-24 出版日期:2013-07-28 发布日期:2013-08-17
通讯作者: 姜振宇（Tel:0431-88782060,E-mail:jlujzy@yhaoo.com.cn） E-mail:jlujzy@yhaoo.com.cn
作者简介:宋晓燕（1981-），女，吉林省磐石市人，主治医师，医学硕士，主要从事肿瘤疾病的基础与临床研究。
基金资助:
吉林省科技厅科研基金资助课题（20090454）


Changes of peripheral blood CD4+CD25+ regulatory T
cells in patients with primary pancreatic cancer before and after
treatment of high intensity focused ultrasound and significance

SONG Xiao-yan¹,MA Ning²,JIANG Zhen-yu²,ZHANG Yan-dong²,DU Bing-yang¹

1.Center of Tumor Diagnosis and Treatment,Center Hospital of Jilin City,Jilin
Province,Jilin 132011,China;2.Department of Rheumatology,First Hospital,Jilin University,Cha
ngchun 130021,China

Received:2013-01-24 Online:2013-07-28 Published:2013-08-17

摘要/Abstract

摘要：

目的：探讨原发性胰腺癌患者接受高强度聚焦超声(HIFU)治疗前后外周血CD4+
CD25+调节性T细胞的变化,阐明HIFU治疗对原发性胰腺癌患者免疫功能的影响。方
法：原发性胰腺癌组患者36例，对照组体检健康者28例，于HIFU治疗前、治疗4周后分别抽取外周静脉血，采
用流式细胞术检测外周血中CD4+CD25+调节性T细胞的百分比，并比较治疗前后原发性胰
腺癌患者KPS评分和癌胚抗原（CEA）、糖类抗原199（CA-199）水平的变化。结果： HIFU治疗前原发性胰腺癌组患者
外周血 CD4+ CD25+ 调节性T细胞百分比高于对照组,差异有统计学意义（P<0.01）；原发性胰腺癌组患者经HIFU治疗4周后外周血CD4+CD25+调节性T细胞百分比较治疗前明显下降,差异有统计学意义（P<0.01）。原发性胰腺癌组患者治疗后KPS评分与治疗前比较明显上升，差异有统计学意义（P<0.05）；CEA和CA-199水平与治疗前比较均明显下降，差异有统计学意义（P<0.05）。结论：HIFU治疗可以改善原发性胰腺癌患者的细胞免疫功能。

关键词: 高强度聚焦超声, 胰腺肿瘤, CD4+CD25+调节性T细胞

Abstract:

Objective To investigate the changes of peripheral blood CD4+CD25+
regulatory T cells in patients with primary pancreatic cancer before and after
treatment of high intensity focused ultrasound(HIFU),and to elucidate the effects of HIFU treatment on immune fun
ction of the patients with primary pancreatic cancer. Methods
Flow cytometry was used to detect the percentage
of CD4+CD25+ regulatory T cells in peripheral blood of the patients
in pancreatic carcinoma group(n=36) before and 4 weeks after HIFU treatment and healthy persons in control group(n=28).The KPS scores,the levels of CEA
and CA-199 of the patients with primary pancreatic cancer before and after treatment were compared.
Results The percentage of CD4+CD25+ regulatory T cells of the patients before HIFU treatment was significantly higher than that in control group,the difference was statistically significant (P<0.01).The percentage of CD4+CD25+ regulatory T cells of the patients in pancreatic cancer group 4 weeks after HIFU treatment was lower than before treatment,the difference was statistically significant (P<0.01).The KPS score of 36 patients with primary pancreatic cancer after HIFU treatment was significantly increased compared with before treatment,the difference was statistically significant ( P<0.05 ).The
levels of CEA and CA-199 of the patients after HIFU treatment was significantly decreased compared with before treatment,the difference was statistically significant (P<0.05).Conclusion HIFU treatment can improve the cell immune function of the patients with pancreatic cancer.


Key words: high intensity focused ultrasound, pancreatic neoplasms, CD4+CD25+ regulatory T cells

中图分类号:

R735.9

宋晓燕，马宁，姜振宇，张彦东，杜冰洋. 原发性胰腺癌患者高强度聚焦超声治疗前后外周血CD4+CD25+
调节性T细胞的变化及其意义[J]. 吉林大学学报(医学版), 2013, 39(4): 791-794.

SONG Xiao-yan,MA Ning,JIANG Zhen-yu,ZHANG Yan-dong,DU Bing-yang. Changes of peripheral blood CD4+CD25+ regulatory T
cells in patients with primary pancreatic cancer before and after
treatment of high intensity focused ultrasound and significance[J]. Journal of Jilin University Medicine Edition, 2013, 39(4): 791-794.

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