冷冻方法和冷冻保护剂对精子活率和尾部低渗肿胀率的影响

doi:吉林省科技厅

冷冻方法和冷冻保护剂对精子活率和尾部低渗肿胀率的影响

王洪亮¹,高元奇²，孙妍²,薛百功²,刘睿智^2*

1. 吉林大学第一医院泌尿外科，吉林长春130021；2. 吉林大学基础医学院细胞生物学教研室，吉林长春130021

收稿日期:2004-10-26 修回日期:1900-01-01 出版日期:2005-07-28 发布日期:2005-07-28
通讯作者: 刘睿智

Effects of cryopreservative methods and cryopreservative mediums on sperm viability and hypotonic swelling rate of human spermatozoa tail

WANG Hong-liang¹,GAO Yuan-qi²,SUN Yan²,XUE Bai-gong²，LIU Rui-zhi^2*

1. Department of Urology, First Hospital, Jilin University, Changchun 130021, China; 2.Department of Cell Biology, School of BasicMedical Sciences, Jilin University, Changchun 130021, China

Received:2004-10-26 Revised:1900-01-01 Online:2005-07-28 Published:2005-07-28
Contact: LIU Rui-zhi

摘要/Abstract

摘要： 目的：探讨冷冻方法和冷冻保护剂类型对精子活率和尾部低渗肿胀率的影响。方法：16例男性不育患者精液分别于冷冻前、加入甘油-卵黄-枸橼酸钠(GYC)和甘油两种冷冻液以快速和慢速冷冻6个月后，采用伊红染色分析精子活率和尾部低渗肿胀率。结果：冷冻后精子活率和尾部低渗肿胀率明显低于冷冻前(P<0.001, P<0.05)；GYC和甘油两种冷冻保护剂对精子活率和尾部低渗肿胀率的影响差异无显著性(P>0.05)；速冻和缓慢冷冻对精子活率和尾部低渗肿胀率的影响差异亦无显著性（P>0.05）。结论：冷冻后精子活率和尾部低渗肿胀率明显降低，不同冷冻方法和冷冻保护剂类型对精子活率和尾部低渗肿胀率无影响。

关键词: 精子尾, 低渗肿胀率, 冷冻保护剂, 精子活率

Abstract: Objective To study the effects of cryopreservative methods and cryopreservative mediums on hypotonic swelling rate of human spermatozoa tail and sperm viability. Methods Human semen samples from 16 male patients with infertility were cryopreserved by fast frozen and slow frozen methods. The hypotonic swelling rate of human spermatozoa tail and sperm viability were analyzed by Eosin staining. Results Post-thawing the spermatozoa tail hypotonic swelling rate and sperm viability were significantly lower than that before (P<0.05, P<0.001), whereas there were no statistical differences in the hypotonic swelling rate of human spermatozoa tail and sperm viability between fast frozen and slow frozen(P>0.05). There were no statistical differences in the hypotonic swelling rate of human spermatozoa tail and sperm viability between GYC and glycerol groups (P>0.05). Conclusion Cryopreservation induces the descent of hypotonic swelling rate of human spermatozoa tail and sperm viability. Cryopreservative methods and cryoprotective mediums could not affect hypotonic swelling rate of human spermatozoa tail and sperm viability. Key words：semen preservation; sperm tail; hypotonic swelling rate; cryoprotective mediums; sperm viability

Key words: sperm tail, hypotonic swelling rate, cryoprotective mediums, sperm viability

中图分类号:

R321.1

王洪亮,高元奇，孙妍,薛百功,刘睿智. 冷冻方法和冷冻保护剂对精子活率和尾部低渗肿胀率的影响[J]. J4, 2005, 31(4): 605-607.

WANG Hong-liang,GAO Yuan-qi,SUN Yan,XUE Bai-gong，LIU Rui-zhi. Effects of cryopreservative methods and cryopreservative mediums on sperm viability and hypotonic swelling rate of human spermatozoa tail[J]. J4, 2005, 31(4): 605-607.