关键词: 一氧化氮合酶, 内皮生长因子, 内皮, 血管, 原位杂交, 免疫组织化学

Abstract: Objective To investigate inducible nitric-oxide synthase (iNOS) and vascular endothelial growth factor (VEGF) expressions in lung cancinoma tissues and their relationships with angiogenesis. Methods The expressions of iNOS mRNA and protein, VEGF and tumor microvascular density (MVD) were detected in 41 lung carcinoma, 10 peri-carcinoma and 6 normal tissues by the methods of in situ hybridization and immunohistochemistry. Meanwhile, their correlations with angiogenesis and tumor clini cal parameters were systematically discussed and analyzed. Results The expressions of iNOS mRNA and protein in lung carcinoma tissues were significantly higher (60.98% and 58.54%，respectively)than those of peri-carcinoma tissues(20%) and normal tissues(16.67%)(P<0.005), which associated with lymph node metastasis and clinical stages (P<0.05). There was obviously positive correlation between iNOS mRNA, iNOS protein and VEGF (r=0.412 5,P<0.01; r=0.462 3, P<0.005). Both iNOS and VEGF are associated with MVD (P<0.05). Conclusion iNOS expression can be used to evaluate clinical stages and predict the risk of metastasis of lung carcinoma. iNOS and VEGF may exert dependable interactions in angiogenesis.

Key words: nitric-oxide synthase, endothelial growth factor, endothelium, vascular, in situ hybridization, immunohisto chemistry