吉林大学学报(医学版)

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碳纤维增强型聚醚醚酮植入下颌骨单层骨皮质缺损模型白兔体内后的生物相容性评价

刘瑞,李明贺,及昕,韩成敏   

  1. 吉林大学口腔医院口腔颌面外科,吉林 长春 130021
  • 收稿日期:2013-10-28 出版日期:2014-05-28 发布日期:2014-06-05
  • 通讯作者: 韩成敏(Tel:0431-85579361,E-mail:han_cm58@sohu.com) E-mail:han_cm58@sohu.com
  • 作者简介:刘 瑞(1987-),女,河南省扶沟县人,医学硕士,主要从事口腔颌面外科学方面的研究。
  • 基金资助:

    吉林省科技厅科技发展计划项目资助课题 (20091113)

Evaluation on biocompatibility of carbon-fiber-reinforced polyether-ether-ketone after implantation  in rabbit models with single bone cortex defect of mandibula

LIU Rui,LI Ming-he,JI Xin,HAN Cheng-min   

  1. Department of Oral and Maxillofacial Surgery,Stomatology Hospital,Jilin University,Changchun 130021,China
  • Received:2013-10-28 Online:2014-05-28 Published:2014-06-05

摘要:

目的:测定碳纤维增强型聚醚醚酮(CF/PEEK)的力学性能,评价其植入下颌骨后的生物相容性及生物活性。方法:将27只成年日本大耳白兔随机分为实验组(9只)和对照组(18只),建立双侧下颌骨单层骨皮质缺损模型。实验组白兔双侧下颌骨植入长条形CF/PEEK复合材料,对照组白兔左侧下颌骨植入自体骨(阳性对照组),右侧不植入任何材料(空白对照组),分别于术后8、12和16周处死白兔(每组3只),截取缺损区颌骨,对标本的材料-骨界面进行大体、影像学和光镜观察,并进行局部组织学评分。结果:大体观察,白兔术后均未出现感染及二次骨折。影像学观察,实验组及阳性对照组白兔颌骨缺损区有明显骨痂形成,修复效果好于空白对照组。组织学观察,与术后第8周比较,术后第12周实验组、阳性对照组和空白对照组局部细胞免疫定量评分明显减小(P<0.01),第16周时最小(P<0.05);实验组、阳性对照组和空白对照组在相同时间点的局部免疫细胞评分比较差异无统计学意义(P>0.05)。第16周时实验组材料周围及阳性对照组缺损区有大量骨小梁形成和成骨细胞散布于骨小梁周围。空白对照组骨缺损处由大量纤维结缔组织填充。随观察时间的延长,实验组及阳性对照组成骨率增高,优于空白对照组(P<0.01)。结论:CF/PEEK复合材料有较好的力学性能,植入白兔下颌骨后免疫反应小,且具有良好的生物相容性及生物活性。

关键词: 聚醚醚酮, 碳纤维, 生物相容性, 骨缺损

Abstract:

Abstract:Objective To investigate the mechanical properties of carbon-fiber-reinforced polyether-ether-ketone(CF/PEEK) composite materials and to evaluate the biocompatibility and bioactivity of CF/PEEK after implanted into mandibula.  Methods 27 adult Japanese white rabbits were randomly divided into experimental group (n=9) and control group (n=18),CF/PEEK was implanted into bilateral mandibula of the rabbits in experimental group,autologous bone was implanted into left mandibula of the rabbits in  positive control group(n=9) while the right sides were taken as blank control group(n=9) without implants.The materials-bone interface of samples were collected to make the observation of gross, imaging and histology and to analyze the  histological score after 8,12,and 16 weeks. Results All the animals and  no infection  had secondary fractures.Obviously callus formation was observed on the edge of the defect in both experimental group and positive control group in the imaging observation,while the rabbits in blank control group had  no obvious change. Compared with 8 weeks after operation,the score evaluation of local cellular immune response showed significant decreasing in  12 weeks after operation(P<0.01),the data was the  minimum in 16 weeks(P<0.05).At the same time,the score evaluation of local cellular immune response in three groups showed no significant difference (P> 0.05).Trabecular bone and osteoblasts could be observed in the bone defect of both experimental group and positive control group after 16 weeks,and blank control group was filled with fibrous connective tissue.The osteogenesis rates  in experimental group and positive control group were increased with the prolongation of time,and they were better than that in blank control  group(P<0.01). Conclusion CF/PEEK material has good  mechanical property,biocompatibility and bioactivity;and it has light immune response after implantation.

Key words: polyetheretherketone, carbon fiber, biocompatibility, bone defect

中图分类号: 

  • R782.2