关键词: 帕金森病, 胶质细胞, 胶质纤维酸性蛋白, 整合素超家族成员Ⅰ型跨膜蛋白, 咪多吡

Abstract:

Abstract:Objective
To discuss the influence of eldepryl on the expressions of glial fibrillary acidic protein (GFAP) and cd11b in substantia nigra and striatum in the rats with Parkinson’s disease (PD),and to clarify the regulatory role of eldepryl in the gliacytes.Methods 72  SD rats were randomly divided into control group,PD model group and eldepryl group,and each group was divided randomly into 4 d and 8 d subgroups (n=12) after the success of model preparation.The PD rat models were established by injecting rotenone in subcutaneous.The number of GFAP and cd11b positive cells and the expressions of GFAP and cd11b were detected by immunohistochemistry and Western blotting method．Results The GFAP and cd11b positive cells were all in a resting state in control group,the GFAP-positive cell body was slender and irregular and had elongated protrusions;the cd11b-positive cell body was small and   branch-like,and it had more slender protrusions.The GFAP and cd11b positive cells were all in a active state in model group,the GFAP-positive cell body was hypertrophy,the projections increased thickening;the cd11b-positive cell body was more bigger,the projections were shorter and thicker,and the number was increased.Compared with model group,the GFAP-positive cell body and protrusions were more slender,the CD11b-positive cell body was more smaller,the projections were more slender,and the number was decreased in eldepryl group.There were a small amount of expression of GFAP and cd11b positive cells in substantia nigra and striatum in the rats in control group,and there was no significant difference between 8 d group and 4 d group(P>0.05). The number of GFAP and cd11b              positive cells and the protein expression levels were significantly increased in model group compared with  control group(P<0.01);there was more expression in 8 d group compared with 4 d group, but there was no significant difference(P>0.05).The number of GFAP and cd11b positive cells and the protein expression levels  in eldepryl group were significantly reduced compared with model group(P<0.01);there were less expression in 8 d group compared with 4 d group,and there was significant difference(P<0.05).Conclusion There are activation and proliferation of the gliacytes in substantia nigra and striatum in the rats with PD,and eldepryl can inhibit the activation and proliferation of gliacytes.

Key words: Parkinson&rsquo, s disease, gliacytes, glial fibrillary acidic protein, cd11b, eldepryl