邻苯二甲酸二丁酯对成年大鼠睾丸Leydig细胞中Cx43蛋白表达及睾酮分泌的影响

doi:10.13481/j.1671-587x.20150217

吉林大学学报(医学版) ›› 2015, Vol. 41 ›› Issue (02): 295-298.doi: 10.13481/j.1671-587x.20150217

邻苯二甲酸二丁酯对成年大鼠睾丸Leydig细胞中Cx43蛋白表达及睾酮分泌的影响

靳曙光, 张燚, 李泠仪, 李环

北华大学公共卫生学院环境卫生学教研室, 吉林吉林 132013

收稿日期:2014-09-16 出版日期:2015-03-28 发布日期:2015-04-04
通讯作者: 李环, 讲师(Tel:0432-64608343, E-mail:jllihuan@126.com) E-mail:jllihuan@126.com
作者简介:靳曙光(1966-), 男, 吉林省吉林市人, 副教授, 医学博士, 主要从事环境卫生学方面的研究。
基金资助:
吉林省科技厅科技发展计划项目资助课题(20100139,201205038);吉林省教育厅科研项目资助课题(2011129,2014211);北华大学大学生国家级创新项目资助课题(201310201050)

Effects of dibutyl phthalate on expression of Cx43 protein and testosterone excretion in Leydig cells of testicle in adult rats

JIN Shuguang, ZHANG Yi, LI Lingyi, LI Huan

Department of Environmental Hygiene, Institute of Public Health, Beihua University, Jilin 132013, China

Received:2014-09-16 Online:2015-03-28 Published:2015-04-04

摘要/Abstract

摘要：

目的:观察邻苯二甲酸二丁酯(DBP)对成年大鼠睾丸Leydig细胞中连接蛋白43(Cx43)的表达及睾酮分泌的影响,探讨DBP对睾酮的调节是否与Cx43有关联。方法:将SD大鼠无菌脱臼处死,原代培养纯化Leydig细胞,将细胞分为溶剂对照组(0.1%DMSO)、DBP组(50 mg·L^-1 DBP)、Cx43增强剂组(50 mg·L^-1 DBP+10 μmol·L^-1 PGE2)和特异睾酮阻断剂组(40 μmol·L^-1氟他胺),分别处理细胞24 h;放射免疫法测定Leydig细胞睾酮水平;细胞免疫荧光和Western blotting法检测细胞中Cx43蛋白表达。结果:细胞染毒24 h后,与DMSO组比较,DBP组Leydig细胞中Cx43蛋白表达水平和睾酮水平均降低(P<0.05);与DBP组比较,DBP+PGE2组Leydig细胞中Cx43表达水平升高(P<0.05),但睾酮水平变化不明显(P>0.05);与DMSO组比较,氟他胺组Leydig细胞中睾酮水平和Cx43蛋白表达水平均明显降低(P<0.05)。结论:DBP对睾酮分泌及Cx43表达均有影响,Cx43对睾酮合成无抑制作用,而睾酮可反向调节Cx43表达;DBP对Leydig细胞睾酮合成抑制作用并不一定以Cx43为靶点。

关键词: 邻苯二甲酸二丁酯, Leydig细胞, Cx43, 睾酮

Abstract:

Objective To explore the effect of dibutyl phthalate(DBP) on the expression of Cx43 protein and testosterone excretion in the Leydig cells of testicle in the adult rats,and to disscuss whether there is relationship between the regulation of testosterone by DBP and Cx43.Methods After the adult SD rats were killed, the cultured primary pure Leydig cells were divided into solvent control group(0.1%DMSO),DBP group (50 mg·L^-1 DBP),Cx43 enhancer group(50 mg·L^-1 DBP+10 μmol·L^-1 PGE2),and specific testosterone blocker group (40 mg·L^-1 flutamid).After cultured for 24 h,radioimmunoassay was used to measure the levels of testosterone in the Leyding cells; cell immunofluorescence and Western blotting method were performed to detect the expressions of Cx43 protein in the Leydig cells.Results Compared with DMSO group,the level of testosterone and the expression level of Cx43 protein in the Leydig cells in DBP group were decreased after 24 h exposure(P<0.05).Compared with DBP group,the testosterone level in DBP+PGE2 group had no obvious change(P>0.05),but the expression level of Cx43 protein was increased(P<0.05).Compared with DMSO group,the level of testosterone and the expression level of Cx43 protein in flutamide group were decreased significantly(P<0.05).Conclusion DBP has the effects on the Cx43 expression and testosterone excreation,and Cx43 has no inhibitory effect on the synthesis of testosterone.However,testosterone can regulate the expression of Cx43 reversely.Therefore,it's not necessary to take Cx43 as the target of DBP in adjusting the synthesis of testosterone in Leydig cells.

Key words: dibutyl phthalate, Leydig cells, Cx43, testosterone

中图分类号:

R961

靳曙光, 张燚, 李泠仪, 李环. 邻苯二甲酸二丁酯对成年大鼠睾丸Leydig细胞中Cx43蛋白表达及睾酮分泌的影响[J]. 吉林大学学报(医学版), 2015, 41(02): 295-298.

JIN Shuguang, ZHANG Yi, LI Lingyi, LI Huan. Effects of dibutyl phthalate on expression of Cx43 protein and testosterone excretion in Leydig cells of testicle in adult rats[J]. Journal of Jilin University Medicine Edition, 2015, 41(02): 295-298.

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邻苯二甲酸二丁酯对成年大鼠睾丸Leydig细胞中Cx43蛋白表达及睾酮分泌的影响

Effects of dibutyl phthalate on expression of Cx43 protein and testosterone excretion in Leydig cells of testicle in adult rats

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