关键词: 创伤和损伤, 免疫组织化学, 白细胞介素1, 内皮生长因子

Abstract: To investigate the immunohistochemical exprssions of IL-1 and VEGF in wound healing process for possible application in determination of incised wound age.Methods Fifty one male SD rats were divided into 17 groups at random， the rat back skin in thirteen groups was incised，then the rats of these groups were sacraficed for different interval (5,10, 30 min and 1,3,5,7,9, 12 h and 1,3,5 , 7 d);another three groups were used as postmortem control，the incisition wound was performed at 5,10,30 after the rat were sacraficed，rat skin from the group without any additional treatment was used as normal control.Immunohistochemical study on the expressions of IL-1 and VEGF were performed on rats skin incised wounds and postmortem incised wounds，and the normal rat skin . Results In antermortem skin incisions，the positive stain of IL-1 was found as early as 0.5 h after incised and lasted till 5 d in epithelial and macrophages of granullation tissue，the rate of IL-1 positive cells was low in the wound specimens in 3 h，and maximized in the wound specimens at 5 h（49.87%±3.42%）and 5 d（47.56%±7.52%）,then the rate decreased to norml.The positive expression of VEGF was found at 3 h after incision and lasted till 10 d.There were no changes of VEGF and IL-1 in the postmortem incised group compared with normal control group. Conclusion The immunohistochemical expressions of VEGF and IL-1 change regularly according to different wound time，VEGF and IL-1 are considered as potentially useful markers for wound time determination in forensic practice.

Key words: wounds and injuries, immunohistochemistry, interleukin-1, endothelial growth factors