关键词: APP, 蛋白酶抑制药, 肝, 损伤, 急性病, 四氯化碳

Abstract: To observe the protective effects of rhKD/APP on carbon tetrachloride-induced acute hepatic injury and find possible mechanisms of hepatic injury. Methods Sixty mice were randomly divided into 6 groups（n=10）:control,model,pos itive control and rhKD/APP groups with different doses (4.5,9.0,18.0 mg•kg ^-1). Pathologic models of acute hepatic injury in mice caused by carbon tetrachloride were set up and rhKD/APP with different dosage were administered (ip) to mice.Effects of rhKD/APP on hepatic injury were observed by measuring enzyme level (AST and ALT)in serum,hepatic malondialdehyde and morphology of hepatic tissue section . Results Compared with model group， serum ALT and AST concentrations in rhKD/APP groups with different doses were decreased significantly (P<0.01, P<0.05), MAD in hepatic tissue in rhKD/APP groups with different doses were decreased (P<0.01, P<0.05). The pathological results showed that there were inflammatory cells and hepatocellular necrosis in model group， the protective effects of rhKD/APP on hepatic injury showed linear relation to the drug dose. Conclusion rhKD/APP has markedly protective effects on acute hepatic injury induced by carbon tetrachloride.

Key words: APP , protease inhibitors , liver , injuries , acute disease, carbon tetrachloride