关键词: 间充质干细胞, CFU-F

Abstract: To explore the effect of lead acetate on the growth of murine mesenchymal stem cells in vitro. Methods 40.00,60.00 and 100.00 μmol•L^-1 of lead acetate were used in the culture of colony-forming unit-fibroblast(CFU-F),the effect on the rate of colony-forming and the rule of variation were observed. Results The rates of colony-forming were(3.30±0.20), (2.40±0.10) and(1.57±0.21)/10⁵ ,when the doses of lead acetate were 40.00 ,60.00 and 100.00 μmol•L^-1 and there were significant differences compared with control group(4.20±0.20)/10⁵, P<0.05). The rates of colony-forming had significant differences between lead acetate with different doses groups (P<0.05). Dose-effect relationship was analyzed with linear correlation and correlation coefficient（r=-0.960,P<0.01）. Colony cells of control group presented fusiform shape while cells of 40.0 μmol•L^-1 of lead acetate group presented irregularity or round shape cultivated in vitro after 6 d. Conclusion The lead acetate suppresses the rate of colony-forming of CFU-F significantly in vitro and the inhibitory effect increases with the increasing of doses of lead acetate.

Key words: mesenchymal stem cells, CFU-F