老年待输血患者Rh系统表型分型和不规则抗体检测及临床意义

doi:10.13481/j.1671-587x.20160618

吉林大学学报(医学版) ›› 2016, Vol. 42 ›› Issue (06): 1143-1146.doi: 10.13481/j.1671-587x.20160618

老年待输血患者Rh系统表型分型和不规则抗体检测及临床意义

王晓宁¹, 王震², 刘冰¹, 金玉顺³, 张冬霞⁴

1. 吉林大学第一医院输血科, 吉林长春 130021;
2. 吉林大学中日联谊医院皮肤科, 吉林长春 130033;
3. 延边大学附属医院输血科, 吉林延吉 133000;
4. 吉林省肿瘤医院输血科, 吉林长春 130012

收稿日期:2016-07-12 出版日期:2016-11-28 发布日期:2016-12-02
通讯作者: 金玉顺,副主任技师(Tel:0433-2660248,E-mail:jys0411@126.com);张冬霞,主任技师(Tel:0431-85873166,E-mail:dongxia628@163.com) E-mail:jys0411@126.com;dongxia628@163.com
作者简介:王晓宁(1985-),女,吉林省长春市人,医学硕士,技师,主要从事临床检验和输血方面的研究。
基金资助:
吉林省科技厅自然科学基金资助课题（20150101143）

Typing of Rh system phenotypes and detection of irregular antibody of elderly blood transfusion candidates and their significances

WANG Xiaoning¹, WANG Zhen², LIU Bing¹, JIN Yushun³, ZHANG Dongxia⁴

1. Department of Blood Transfusion, First Hospital, Jilin University, Changchun 130021, China;
2. Department of Dermatology, China-Japan Union Hospital, Jilin University, Changchun 130033, China;
3. Department of Blood Transfusion, Affiliated Hospital, Yanbian University, Yanji 133000, China;
4. Department of Blood Transfusion, Jilin Provincial Tumor Hospital, Changchun 130012, China

Received:2016-07-12 Online:2016-11-28 Published:2016-12-02

摘要/Abstract

摘要：

目的：了解老年待输血患者Rh系统表型分型，分析其不规则抗体阳性率，为老年患者临床输血安全性提供依据。方法：选择老年待输血患者587例，采用微柱凝胶法对患者进行Rh系统抗原检测及不规则抗体鉴定。结果：587例患者中测得Rh血型表现型共7种，均为RhD阳性，Rh血型表现型的频率由高到低分别为CCDee占38.7%（227/587）、CcDEe占34.7%（204/587）、CcDee占12.4%（73/587）、ccDEE占8.7%（51/587）、ccDEe占4.6%（27/587）、CCDEe占0.7%（4/587）和ccDee占0.2%（1/587）。587例患者中检出不规则抗体6例，阳性率为1.02%，分别为4例抗-E（占66.67%）、1例抗-c（占16.67%）、1例抗-M（占16.67%）；有输血史或妊娠史患者不规则抗体阳性率[2.65%（5/189）]高于无输血史及妊娠史者[0.25%（1/398）]（χ²=5.11，P<0.05）；不同民族老年患者不规则抗体阳性率比较差异无统计学意义（χ²=0.10，P>0.05）。结论：Rh系统抗体是老年待输血患者中最主要的不规则抗体，应对有输血史或妊娠史的老年患者进行Rh表型分型检测及不规则抗体筛查鉴定，以确保老年患者的输血安全。

关键词: Rh表型, 微柱凝胶法, 不规则抗体

Abstract:

Objective: To understand the typing of Rh system phenotypes and analyze the positive rate of irregular antibody in Rh system,and to provide basis for blood transfusion of the elderly patients in clinic.Methods: A total of 587 elderly blood tranfusion candidates were included. Microcolumn gel test was applied to detect the antigens of Rh system and identify the irregular antibody in Rh system.Results: There were 7 kinds of Rh blood group phenotypes with postive expression of RhD in 587 patients; the positive rates of irregular antibodies from high to low were CCDEE 38.7%(227/587),CcDEe 34.8%(204/587),CcDee 12.4%(73/587),ccDEE 8.7%(51/587),ccDEe 4.6%(27/587) and CCDEe 0.7%(4/587),ccDee 0.2%(1/587).There were 6 kinds of irregylar antibodies in 587 patients;the positive rate was 1.02%,including 4 cases of Anti-E (66.67%),1 case of Anti-c(16.67%),1 case of anti-M(16.67%).The postive rate of irregular antibody in the patients with blood transfution history and pregnancy history (2.65%,51/89) was higher than those without blood transfusion history or pregnancy history 0.25%,13/98)(χ²=5.11,P<0.05). There were no significant differences of the positive rates of irregular antibody between different nationalities(χ²=0.10,P>0.05).Conclusion: The irregular antibody of Rh system is the major antibody in the eldery blood transtusion candidates.The detection of typing of Rh system phenotypes and identification of irregular antibodies in the patients with blood transfusion history and pregnancy history should be performed to ensure the safty of blood transfusion.

Key words: Rh phenotype, microcolum gel test, irregular antibody

中图分类号:

R457.1

王晓宁, 王震, 刘冰, 金玉顺, 张冬霞. 老年待输血患者Rh系统表型分型和不规则抗体检测及临床意义[J]. 吉林大学学报(医学版), 2016, 42(06): 1143-1146.

WANG Xiaoning, WANG Zhen, LIU Bing, JIN Yushun, ZHANG Dongxia. Typing of Rh system phenotypes and detection of irregular antibody of elderly blood transfusion candidates and their significances[J]. Journal of Jilin University Medicine Edition, 2016, 42(06): 1143-1146.

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老年待输血患者Rh系统表型分型和不规则抗体检测及临床意义

Typing of Rh system phenotypes and detection of irregular antibody of elderly blood transfusion candidates and their significances

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