热休克蛋白70在消化性溃疡及急性糜烂出血性胃炎组织中的表达及意义

doi:国家“十五”科技公关计划资助课题(2004BA7

热休克蛋白70在消化性溃疡及急性糜烂出血性胃炎组织中的表达及意义

李玉琴¹，黄民²，王英凯¹，金京兰¹，唐彤宇¹，高沿航¹，王丹¹

(1. 吉林大学第一医院消化内科，吉林长春 130021；2. 吉林大学基础医学院生理学教研室，吉林长春 130021)

收稿日期:2008-01-18 修回日期:1900-01-01 出版日期:2008-09-28 发布日期:2008-09-28
通讯作者: 王英凯

Expressions of HSP70 in peptic ulcer and acute erosive hemorrhagic gastritis and their significances

LI Yu-qin¹,HUANG Min²,WANG Ying-kai¹, JIN Jing-lan¹, TANG Tong-yu¹, GAO Yan-hang¹,WANG Dan¹

(1.Department of Gastroentrology, First Hospital, Jilin University, Changchun 130021, China;2. Department of Physiology, School of Basic Medical Sciences, Jilin University, Changchun 130021,China）

Received:2008-01-18 Revised:1900-01-01 Online:2008-09-28 Published:2008-09-28
Contact: WANG Ying-kai

摘要/Abstract

摘要： 目的：研究热休克蛋白70(HSP70）在消化性溃疡及急性糜烂出血性胃炎患者治疗前、治疗后不同阶段及不同时期的表达，探讨其与消化性溃疡和急性糜烂性胃炎的关系。方法：利用纤维电子胃镜检查选取消化性溃疡活动期患者75例（胃溃疡29例，十二指肠溃疡46例）及急性糜烂出血性胃炎13例，正常胃黏膜组26例，前两组患者进行抗溃疡治疗。用RT-PCR方法检测其治疗前后HSP70 及内参GAPDH基因表达，用各样品HSP70与内参GAPDH的积分吸光度比值来表示HSP70 mRNA的表达量。结果：消化性溃疡患者HSP70 mRNA的表达水平明显高于正常者(P<0.01)；治疗后消化性溃疡各期（活动期、愈合期、瘢痕期）HSP70 mRNA的表达水平高于治疗前(P<0.05或P<0.01)；而治疗后消化性溃疡各期（活动期、愈合期、瘢痕期）及不同时期（治疗后第1个月、第2个月）之间差异无显著性(P>0.05)。急性糜烂出血性胃炎患者HSP70 mRNA表达水平高于正常对照(P<0.01)；急性糜烂出血性胃炎各期（好转组和痊愈组）治疗后第1个月的HSP70 mRNA的表达水平高于治疗前 (P<0.01或P<0.05)；而好转组和痊愈组比较差异无显著性(P>0.05)。结论：HSP70作为一种应激蛋白，参与了消化性溃疡及急性糜烂性胃炎的修复。

关键词: 消化性溃疡, 急性糜烂出血性胃炎, 逆转录聚合酶链反应

Abstract: To study the relationship between heat shock protein 70 (HSP70) and the pathogenesis of peptic ulcer disease, acute erosive hemorrhagic gastritis. Methods Three groups were selected by conducting endoscopy: 75 patients with peptic ulcer at active stage (29 cases of gastric ulcer, 46 cases of duodenal ulcer), 13 patients 　with acute erosive hemorrhagic gastritis, 26 cases of normal gastric mucosa normal, and the patients in the first two groups were given antiulcer therapy.The expressions of HSP70 and GAPDH mRNA before and after treatment were detected by reverse transcription polymerase chain reaction (RT-PCR). Results The expression level of HSP70 mRNA in ulcer group was significantly higher than that in normal group (P<0.01).The expression levels of HSP70 mRNA in ulcer group at three stages (active, healing, and scar stages)after treatment were significantly higher than those before treatment respectively(P< 0.01 or P<0.05).Nevertheless,after treatment, there were no significant differences between various stages(active, healing, and scarring stage) of ulcer group and also between various periods(one month, two months) at each stage(P>0.05).The expression level of HSP70 mRNA in gastritis group was higher than that in normal group(P<0.01).In gastritis group(improved group and healing group),the expression levels of HSP70 mRNA one month after treatment were higher than those before treatment(P<0.01 or P<0.05).Conclusion HSP70 as a stress protein,participates in repair of peptic ulcer and acute erosive hamorrhagic gastritis.

Key words: peptic ulcer, acute erosive hemorrhagic gastritis, reverse transcription polymerase chain reaction

中图分类号:

R573

李玉琴，黄民，王英凯，金京兰，唐彤宇，高沿航，王丹. 热休克蛋白70在消化性溃疡及急性糜烂出血性胃炎组织中的表达及意义[J]. J4, 2008, 34(5): 846-849.

LI Yu-qin,HUANG Min,WANG Ying-kai, JIN Jing-lan, TANG Tong-yu, GAO Yan-hang,WANG Dan. Expressions of HSP70 in peptic ulcer and acute erosive hemorrhagic gastritis and their significances[J]. J4, 2008, 34(5): 846-849.

[1]	董泽丰, 郑田利, 范雪佳, 裴晓方. 2011-2013年成都地区发热呼吸道症候群住院患儿鼻病毒流行病学特征[J]. 吉林大学学报(医学版), 2016, 42(01): 177-181.
[2]	刘洁, 贾天军, 刘雪晴, 贾晓辉, 翟雪琼. 人甘露糖结合凝集素相关丝氨酸蛋白激酶-2 C端基因的克隆与鉴定[J]. J4, 2011, 37(1): 51-55.
[3]	宋明旭, 李莉华, 丁苏宁, 刘志辉, 周希科. PDGF家族在原发性胃腺癌组织中的表达及其意义[J]. J4, 2011, 37(1): 97-101.
[4]	张晓霞, 孙晓琦, 张松灵, 高咏梅, 田海涵. 卵巢浆液性囊腺癌组织中β-连环蛋白的表达及其意义[J]. J4, 2010, 36(3): 536-539.
[5]	张晓霞，费军伟，舒畅，倪劲松，王芯蕊，李荷莲. 卵巢浆液性囊腺癌组织中prostasin基因的表达及意义[J]. J4, 2009, 35(2): 280-283.
[6]	张宸豪，柳忠辉，刘海岩，王轶楠，葛敬岩，于昉，台桂香. 激活素A及其结合蛋白在STZ损伤小鼠胰腺组织中的表达及意义[J]. J4, 2009, 35(2): 292-295.
[7]	汲坤，王波，李馨，于浩，张灵，胡嘉弟，赵雪俭，李扬. 细胞死亡调节因子GRIM-19蛋白在肾脏透明细胞癌组织中的表达及意义[J]. J4, 2009, 35(1): 146-149.
[8]	马舒贝，孙威，李霞，刘亢丁. 大鼠局灶性脑缺血后脑组织Ang-1和Ang-2 mRNA的表达及意义[J]. J4, 2009, 35(1): 30-33.
[9]	张晓霞，费军伟，于晓伟，倪劲松，王芯蕊，李荷莲. 卵巢癌组织中PI3K的表达及意义[J]. J4, 2008, 34(6): 1027-1030.
[10]	李光虎，刘伟，付彤，刘国津. muc-1基因在非小细胞肺癌组织中的表达及临床意义[J]. J4, 2008, 34(6): 1046-1049.
[11]	牟旭鹏，孔宁，申茉函，韩东，张林，颜炜群. 人碱性成纤维细胞生长因子cDNA的克隆及在毕赤酵母中的表达[J]. J4, 2008, 34(5): 755-758.
[12]	张晓霞，王影，黄蓉，李相军，任立群，李荷莲. 人激肽释放酶10在卵巢癌组织中的表达及其意义[J]. J4, 2008, 34(4): 661-664.
[13]	李然伟，王珂,杨泽成,刘永红，吕佳音，吴丹华，赵燕颖. siRNA抑制人前列腺癌PC-3细胞株VEGF的表达及细胞增殖[J]. J4, 2008, 34(4): 585-589.
[14]	陈建芳，胡晓春，张颖丽. 基质金属蛋白酶8 mRNA在根尖肉芽肿和根尖囊肿组织中的表达及其意义[J]. J4, 2008, 34(3): 466-468.
[15]	吕佳音，吴丹凯，徐春华，张舵舵，吴丹华，高忠礼，赵燕颖. siRNA对骨肉瘤细胞MDM2的表达及细胞增殖的抑制作用[J]. J4, 2008, 34(3): 442-445.