关键词: 糖耐量异常, 氧化作用, 非酶糖基化

Abstract: To study the influence of dihydromyricetin on the oxidation and non-enzymatic glycosylation in impaired glucose tolerance rats.Methods Animal model of impaired glucose tolerance rat was built by intragastrical(i.g.) injection of D-galactose generally.After 8 weeks intragastrical(i.g.) injection of dihydromyricetin(experimental group) and metformin (positive control),the levels of fasting blood glucose and tow-hour postprandial blood glucose,the insulin level and its resistance index,the levels of glycosylated hemoglobin (GHb) and advanced glycation end-products(AGEs) were detected,and the activities of superoxide dismutase(SOD) and glutathione peroxidase (GSH-Px),the content of malondiadehyde(MDA) were determined respectively. Results Compared with model group，dihydromyricetin decreased the level of two-hour postprandial blood glucose (P<0.05),lowered serum insulin level, its resistance index (P<0.05) and the contents of MDA and AGEs in serum (P<0.05) . In addition,it increased the activity of GSH-Px in serum (P<0.05); but there was no difference of both the content of GHb and the activity of SOD between all groups(P>0.05).Conclusion Dihydromyricetin can significantly improve the state of impaired glucose tolerance rats; the mechanism may be related to its effect of improving the antioxidant activity of GSH-Px,inhibiting non-enzymatic glycation,lowering body oxidative stress and insulin resistance and promoting the use of blood glucose in peripheral organizations.

Key words: impaired glucose tolerance, oxidation, non-enzymatic glycosylation