关键词: 再灌注损伤, 高脂血症, 大鼠, Wistar

Abstract: Abstract:Objective To investigate the protective effect of Schizandrae Lignanoid (SCL) on myocardial ischemia reperfusion injury in hyperlipidemic rats and its mechanisms and provide theoretical basis and experiment foundation for treatment of myocardial ischemia complicated with hyperlipidemic disease with SCL in the future. Methods After hyperlipidemic rat models were set upby oral administration of high lipid emulsion,the rats were divided into seven groups: control group，sham group，model group,SCL 60，20，5 mg•kg^-1 groups and 200 mg•kg^-1 CDT group.After reperfusion,the blood samples taken from the ventricles were assayed for blood lipid;MPO activities of ischemic myocardium were measured;Infarct area of left ventricles was measured by Evens blue-TTC staining,and myocardial pathological changes were also observed.Results Compared with model group, the myocardial infarct area/ventricle area ratios and MPO activities in SCL 60，20，5 mg•kg^-1 groups decreased (P<0.01) with the raise of SCL dose;In SCL 60 and 20 mg•kg^-1 groups myocardial fiber rupture and necrosis in ischemic area with less focal hemorrhage and inflammatory cell infiltration decreased;In SCL 60 and 20 mg•kg^-1 groups the levels of serum TC,TG,LDL-c,ApoB decreased and HDL-c,ApoA1 increased (P<0.05 or P<0.01）.Conclusion SCL has protective effects on myocardial ischemia reperfusion injury in hyperlipidemic rats and the mechanisms involved may be related to its regulation of blood lipid and inhibition of neutrophil infiltration.

Key words: reperfusion injury, hyperlipidemia, rats, Wistar