放线共生放线杆菌的分离、鉴定及耐药谱的测定

doi:吉林省科研发展计划项目

放线共生放线杆菌的分离、鉴定及耐药谱的测定

李毅¹，杨涛²，孙延波³，关显智³，黄洋¹，孙宏晨¹

1. 吉林大学口腔医院口腔内科，吉林长春130041；2. 长春市儿童医院口腔科，吉林长春130051；3. 吉林大学基础医学院病原微生物学教研室，吉林长春130021

收稿日期:2003-09-25 修回日期:1900-01-01 出版日期:2004-03-28 发布日期:2004-03-28

Identification of Actinobacillus actinomycetemcomitansand its spectrum of resistance to antibiotics

LI Yi¹,YANG Tao²，SUN Yan-bo³，GUAN Xian-zhi³，HUANG Yang¹，SUN Hong-chen¹

1. Department of Periodontitis, Stomatology Hospital,Jilin University,Changchun 130041,China;2.Department of Stomatology,Changchun Pediatrics Hospital,Changchun 130051,China;3.Department of Microbiology,School of Basic Medical Sciences,Jilin University,Changchun 130021,China

Received:2003-09-25 Revised:1900-01-01 Online:2004-03-28 Published:2004-03-28

摘要/Abstract

摘要： 目的：探讨放线共生放线杆菌（A.a）分离、鉴定的方法，确定其耐药谱。方法：采用选择性培养基、厌氧培养技术及生化鉴定的方法从临床青少年牙周炎、成人快速进展型牙周炎及青少年严重牙龈炎患者的牙周袋内分离出A.a。在此基础上进行了10种抗生素对所分离的全部A.a菌株及1株标准菌株最低抑菌浓度的检测。结果：共分离出24株A.a，其对庆大霉素、四环素、氨苄青霉素、丁氨卡那霉素及青霉素G呈现出不同程度的耐药性，但对罗红霉素、甲硝唑、链霉素、红霉素及头孢唑啉钠敏感。结论：罗红霉素、甲硝唑、链霉素、红霉素及头孢唑啉钠在治疗与A.a相关的牙周病能够发挥作用，本研究为A.a耐药性质粒的提取及鉴定打下了基础。

关键词: 抑菌浓度

Abstract: Objective To study the method to isolate and identify Actinobacillus actinomycetemcomitans (A.a) and insure its resistance to antibiotics. Methods A.a was isolated from specimens of human periodontal disease, and identification of A.a was carried out by anaerobic cultivation, observation of growth properties on TSBV and systematically biochemical reaction; and the spectrum of A.a′s resistance to antibiotics was identified. Results Twenty-four strains of A.a were acquired and identified; They were sensitive to Re，MTZ，Sm，Em，and Cef. Conclusion Re，MTZ，Sm，Em, and Cef can play a important role in treatment of peridontitis.

Key words: bacteriostatic concentration

中图分类号:

R379.1

李毅，杨涛，孙延波，关显智，黄洋，孙宏晨. 放线共生放线杆菌的分离、鉴定及耐药谱的测定[J]. J4, 2004, 30(2): 227-229.

LI Yi,YANG Tao，SUN Yan-bo，GUAN Xian-zhi，HUANG Yang，SUN Hong-chen. Identification of Actinobacillus actinomycetemcomitansand its spectrum of resistance to antibiotics[J]. J4, 2004, 30(2): 227-229.