磷酸钙骨水泥复合骨形态发生蛋白对兔实验性股骨头缺血性坏死的修复作用

doi:中日国际合作研究项目(2003)

磷酸钙骨水泥复合骨形态发生蛋白对兔实验性股骨头缺血性坏死的修复作用

苏云¹，张伟¹，胡春明^1*，徐爽²，张贵雨¹，吕涛¹，武首先¹

1.吉林大学第一医院骨科，吉林长春 130021；2. 解放军第208医院麻醉科，吉林长春 130062

收稿日期:2004-10-18 修回日期:1900-01-01 出版日期:2005-09-28 发布日期:2005-09-28
通讯作者: 胡春明

Repair effects of calcium phosphate cement as carrier to bonemorphogenetic protein on experimental avascular necrosis of femoral head in rabbits

SU Yun¹, ZHANG Wei¹, HU Chun-ming^1*, XU Shuang², ZHANG Gui-yu¹, LU Tao¹, WU Shou-xian¹

1. Department of Orthopedics, First Hospital, Jilin University, Changchun 130021, China；2. Department of Anesthesiology, No.208 Hospital of PLA, Changchun 130062, China

Received:2004-10-18 Revised:1900-01-01 Online:2005-09-28 Published:2005-09-28
Contact: HU Chun-ming

摘要/Abstract

摘要： 目的：观察自制CPC/BMP复合人工骨对兔实验性股骨头缺血性坏死（ANFH）的修复作用，并探讨其临床应用的可行性。方法:24只健康成年兔分成CPC组12只和CPC/BMP组12只，在兔左侧股骨头建立ANFH骨缺损模型。将CPC作为BMP的载体制备成CPC/BMP复合材料后，植入骨缺损处，同时设立单纯CPC填充对照组。术后3及12周分批处死动物，每组每次处死6只。通过大体观察、X线摄片、HE染色、透射电镜等手段观察新骨形成和材料降解情况。综合评价CPC/BMP复合材料对ANFH骨缺损的修复能力。结果：CPC/BMP组3及12周时成骨情况明显优于单纯CPC组。CPC/BMP组3周时，CPC表面及内部可见较多新骨及幼稚骨细胞，CPC已被分解成多个区域（岛状分布），有板层骨形成；透射电镜观察可见成骨细胞侵入CPC内部，细胞浆内粗面内质网及线粒体增多扩张。CPC/BMP组12周时，光镜下见CPC降解，新生骨长入材料并与之相互分割包裹，骨小梁增粗； X线片显示新骨显影较清，材料密度减低，吸收降解现象明显；透射电镜观察可见大量成骨细胞散在于CPC颗粒之间，细胞内粗面内质网及线粒体增多扩张，较多毛细血管侵入材料内部。在材料降解速度方面，CPC/BMP组明显快于CPC组。结论：CPC是BMP的理想载体，CPC/BMP复合材料具有较强的传导成骨和诱导成骨活性，对实验性ANFH具有修复作用。在降解速度上CPC/BMP组明显快于单纯CPC组。

关键词: 骨粘合剂, 磷酸钙类, 生物相容性材料, 股骨头坏死

Abstract: Objective To observe the repairing effects of a new type of bone graft material constructed by combining calcium phosphate cement (CPC) with bone morphogenetic protein (BMP) on avascular necrosis of femoral head (ANFH) in rabbits; to evaluate the feasibility to use this material to repair ANFH in clinic. Methods The models of bone defect of ANFH were established in the left femoral heads in 24 healthy adult rabbits, of which 12 defects were implanted with CPC/BMP composite, 12 implanted with CPC. The specimens were harvested separately at the end of 3 and 12 weeks after operation (each time 6 rabbits in each group). In order to observe the formation of new bone and the degradation process of the material, a series of examinations were carried out, such as radiograph, histomorphology, transmission electron microscope (TEM). The results in CPC group and CPC/BMP group were compared on the same condition. Results New bone formation in CPC/BMP group was superior to that in CPC group significantly at every stage. In CPC/BMP group, at the 3rd week, new bone and many puerile osteocytes could be found on the surface or inside of CPC, which had been divided into many small parts like islands, with Lamellar bone forming; under TEM, osteoblasts could be found invading CPC, whose rough endoplasmic reticulum (RER) and mitochondrion in plasma increased and swelled. At the 12th week in CPC/BMP group, new bone invaded CPC and enwrapped each other, with trabeculae enlarging; the image of new bone was clear by X-ray examination, with the density of CPC decreased; under TEM, many osteoblasts could be found spreading in CPC, whose RER and mitochondrion in plasma increased and swelled; many capillaries could be found in CPC. The speed of degradation in CPC/BMP group was evidently higher than that in CPC group. Conclusion CPC is an ideal carrier to BMP. The CPC/BMP composite has strong osteoconductibility and osteoinductibility and great potential in ANFH repairing. The speed of degradation of CPC/BMP is evidently higher than that of CPC.

Key words: bone cements, calcium phosphates, biocompatible materials, femur head necrosis

中图分类号:

R687.3

苏云，张伟，胡春明，徐爽，张贵雨，吕涛，武首先. 磷酸钙骨水泥复合骨形态发生蛋白对兔实验性股骨头缺血性坏死的修复作用[J]. J4, 2005, 31(5): 684-687.

SU Yun, ZHANG Wei, HU Chun-ming, XU Shuang, ZHANG Gui-yu, LU Tao, WU Shou-xian. Repair effects of calcium phosphate cement as carrier to bonemorphogenetic protein on experimental avascular necrosis of femoral head in rabbits[J]. J4, 2005, 31(5): 684-687.

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