关键词: 辐射效应, 腹腔, 辐射效应, 转录因子

Abstract: Objective To study the effect of different doses of X-irradiation on transcription factor NF-κB in peritoneal macrophages of mice. Methods Electrophoresis mobility shift assay (EMSA) was used to separate the binding bands of NF-κB with DNA probe, the results were analyzed by Phosphor Imager Scanning System.Results The DNA binding activity of NF-κB heterodimer p65/50 in peritoneal macrophages of irradiated mice increased compared with that of sham-irradiation mice, peaked after irradiation with 0.100 Gy of X-rays, followed by gradually returned to the sham-irradiation level, then increased again after irradiation with 6.000 Gy of X-rays; but the DNA binding activity of NF-κB homodimer p50/50 had no changes after irradiation. Conclusion Irradiation with both low dose and high dose of X-rays can induce the increase of the DNA binding activity of NF-κB predominantly heterodimer p65/50 in peritoneal macrophages in irradiated mice.

Key words: radiation effects, peritoneal, radiation effects, transcription factor