关键词: 眼, 细胞培养, 血小板源性生长因子, 免疫学

Abstract: To study the inhibition of anti-PDGF on proliferation of culturedhuman retinal pigment epithelial cells (hRPE) in vitro.MethodshRPE were cultivated and were exposed to different concentrations of anti-PDGF (0,1×10^-6,5×10^-6,1×10^-5,5×10^-5 and 1×10^-4 mg•L^-1) respectively .Growth curves were measured with cell counting and the vitalities of cells were examined by percentage of vital cells and total cells.Using MTT staining colorimetric to measure the inhibitory rate.The changes of cell cycle of hRPE were collected and their growth were detected with FCM analysis and the morphological changes of cells were observed by light microscope and electron microscope. Results Anti-PDGF of 1×10^-6mg•L^-1 stimulated hRPE proliferation slightly.Anti-PDGF at dosages ranging from 5×10^-6mg•L^-1 to 1×10^-4mg•L^-1 inhibited cell proliferation effectively in a dose-dependent and time-dependent manner (P<0.05).The inhibitory rates of anti-PDGF were 12.55%,43.72%,55.1% and 55.1%.hRPE might be blocked at G₂/M phase in their growth cycles after treated with anti-PDGF (5×10^-5mg•L^-1) for 72 h,and the electron microscopy result showed a significant reduction of microvilli,margination of nuclear chromatin and intact plasmalemma. Conclusion Anti-PDGF can inhibit hRPE in a dose-dependent and time-dependent manner.It’s inhibitory function lies in inducing apoptosis without resulting in cell putrescence.

Key words: cell culture, platelet-derived growth factor, immunology,