关键词: 毛细胞, 内, 毛细胞, 外, 缺氧, 体外培养

Abstract: To establish a practical model for Wistar rat cochlea organ cultivated in vitro and observe the effect of hypoxia on the inner hair cells (IHC) and outer hair cells (O HC). Methods The cochlear basal membrane from 3-day-old Wistar rats was used.The cochlear basal membrane cells were divided into 8 groups.Among them 7 groups were used as experimental groups.The cells in experimental groups were put in incubator (37℃, 90％N₂, 5％CO₂, 5％O₂)to cultivate with hypoxia for different time. The other group was used as control,the cells were cultivated in incubator( 37℃, 5％CO₂).The cultures were phalloidin-labeled and the number of IHC / OHC were counted in unit area （24 mm×36 mm） and the morphological changes of IHC and OHC were observed under microscope. Results No significant change of IHC was found under hypoxia for 0.5 h. IHC under hypoxia for 1 h appeared light swelling and cell density decreased,but the cell density in unit area had no remarkable difference compared with control (P>0.05). IHC loss was found under hypoxia for 2 h to 48 h in a time dependent manner, the cell density in unit area had remarkable difference compared with control (P<0.01). The phenomena of empty nerve cup was found under hypoxia for 6 h. The OHC loss a nd array disorders appeared under hypoxia for 6 h and severe damage under hypoxia for 48 h, the cell density in unit area had remarkable difference compared with control (p<0.01). Conclusion Hypoxia could cause the hair cell loss in vitro. Moreover IHC is more susceptible to hypoxia than OHC.

Key words: inner, hair cell , outer, hypoxia, culture in vitro