胃癌患者血中游离癌细胞CK-20 mRNA检测的临床意义

doi:吉林省科技厅基金资助课题（990568-2）

胃癌患者血中游离癌细胞CK-20 mRNA检测的临床意义

乔士兴，乔昕^*，王文华

吉林大学第二医院普通外科，吉林长春 130041

收稿日期:2006-08-22 修回日期:1900-01-01 出版日期:2007-03-28 发布日期:2007-03-28

Detection of free cancer cell CK-20 mRNA in peripheral blood in patients with gastric carcinoma

QIAO Shi-xing, QIAO Xin^*, WANG Wen-hua

Department of General Surgery, Second Hospital, Jilin University, Changchun 130041，China

Received:2006-08-22 Revised:1900-01-01 Online:2007-03-28 Published:2007-03-28

摘要/Abstract

摘要： 目的：探讨胃癌患者手术前后血中游离癌细胞CK-20 mRNA的检测情况及临床意义。方法：采用RT-PCR方法，检测40例胃癌患者及20例胃、十二指肠良性疾病患者手术前后血中游离癌细胞CK-20 mRNA表达。结果：胃、十二指肠良性疾病组CK-20 mRNA表达均为阴性，胃癌组术后CK-20 mRNA表达阳性率(37.5%，15/40)明显高于术前(22.5%，9/40)（P<0.05）。术中先结扎肿瘤周围回流静脉组（A组）术后CK-20 mRNA表达阳性率为35%，不结扎回流静脉组（B组）术后阳性率为40%，二组比较差异无显著性（P>0.05）。CK-20 mRNA阳性检出率与胃癌分化程度无明显相关性（r=0.012, P>0.05），与Bormann分型亦无明显相关性（r=0.024，P>0.05）。结论：进展期胃癌术前已有部分患者血中存在游离癌细胞，手术中的牵拉刺激可增加癌细胞血行播散的可能性，术中单纯靠结扎回流静脉的方法无法完全阻断癌细胞播散入血。

关键词: 肿瘤循环细胞, CK-20 mRNA

Abstract: Objective To explore the clinical significance of detection of free cancer cell CK-20 mRNA in peripheral blood in patients with gastric carcinoma before and a fter operation.Methods CK-20 mRNA levels of free cancer cells in peripheral blood in 40 patients with gastric cancer and 20 cases of benign disease of stomach and duodenum before and after operation were determined with PCR technique.Results CK-20 mRNA did not express in the benign disease group. The positive expression rate of CK-20 mRNA was 22.5% (9/40) in gastric cancer group before opera tion and the positive rate was 37.5%(15/40) after operation (P<0.05). The positive rate of CK-20 mRNA after operation in group A (peripheral veins were ligated around stomach at first ) was 35%, and in group B ( peripheral veins were not ligated around stomach) was 40%, there was no significan t difference between group A and group B (P>0.05). The positive expression of CK-20 mRNA has negative correlation with degree of differentiation and Bormann typing(r=0.012，P>0.05；r=0.024，P>0.05).Conclusion There are some free cancer cells in the prepheral blood of some patients with gastric cancer before operation. Pulling and stimulating tumor in the operation may increase cancer cell dissemination. Simple ligation of peripheral veins around stomach could not completely prevent cancer cell dissemination in the peripheral blood.

Key words: neoplasm circulating cells, CK-20 mRNA

中图分类号:

R735.2

乔士兴，乔昕，王文华. 胃癌患者血中游离癌细胞CK-20 mRNA检测的临床意义[J]. J4, 2007, 33(2): 341-343.

QIAO Shi-xing, QIAO Xin, WANG Wen-hua. Detection of free cancer cell CK-20 mRNA in peripheral blood in patients with gastric carcinoma[J]. J4, 2007, 33(2): 341-343.