阿霉素诱导亚铁血红素氧化酶-1在大鼠肝、脾和肾脏组织的表达及其对肝功能的影响

doi:吉林省发展计划委员会高科技产业发展项目资

阿霉素诱导亚铁血红素氧化酶-1在大鼠肝、脾和肾脏组织的表达及其对肝功能的影响

张海山¹，张学文¹，马淑荣²，张德恒¹，邱东涛^△

1. 吉林大学中日联谊医院基本外科，吉林长春 130031；2. 吉林大学中日联谊医院肾风湿科，吉林长春130031

收稿日期:2003-01-15 修回日期:1900-01-01 出版日期:2004-01-28 发布日期:2004-01-28

Expression of heme oxygenase-1 induced by doxorubicin in liver，spleen， and kidney and effect of doxorubicin on liver function

ZHANG Hai-shan¹,ZHANG Xue-wen¹, MA Shu-rong²,ZHANG De-heng¹，QIU Dong-tao^△

1. Department of General Surgery, China-Japan Union Hospital, Changchun 130031， China;2. Department of Nephro-Rheumatology, China-Japan Union Hospital, Changchun 130031, China

Received:2003-01-15 Revised:1900-01-01 Online:2004-01-28 Published:2004-01-28

摘要/Abstract

摘要： 目的：观察阿霉素诱导亚铁血红素氧化酶-1（HO-1）在大鼠肝、脾和肾脏组织的表达及对肝功能的影响。方法：雄性Wistar大鼠随机分为空白对照组、小剂量阿霉素组(1 mg•kg^-1) 和大剂量阿霉素组(10 mg•kg^-1)，分别于给药后6、12、18、24和48 h切取阿霉素组与对照组部分肝脏用蛋白电泳法检测HO-1表达，切取注药后48 h阿霉素组和对照组部分肝、脾和肾行HO-1免疫组化染色，同时检测三组大鼠肝功能、血清ET-1和TNF-α。结果：蛋白电泳检测显示HO-1蛋白在正常肝脏内的表达阴性；给药后6 h两组均可检测出HO-1的表达，24 h达到高峰，48 h HO-1表达已明显减弱。免疫组化染色显示，注射阿霉素48 h,肝、脾和肾仍可见大量HO-1染色阳性细胞；对照组肝脏无染色阳性细胞，脾肾可见少量染色阳性细胞。肝功能指标、血清TNF-α和ET-1对照组与小剂量阿霉素组差异无显著性(P>0.05)，大剂量阿霉素组上述指标明显高于对照组和小剂量阿霉素组(P<0.05)。结论：阿霉素可诱导肝脏、脾脏和肾脏HO-1表达，小剂量阿霉素对大鼠肝脏未造成明显损害。

关键词: 阿霉素, 血红素氧化酶(脱环), 生物合成, 肝功能

Abstract: Objective To study the expression of heme oxygenase-1 (HO-1) induced by doxorubicin in liver,spleen, and kidney and the effect of doxorubicin on liver function. Methods Male Wistar rats were randomly divided into control, low-dose (1 mg•kg^-1) and high-dose(10 mg•kg^-1) group. The expressions of HO-1 in liver were detected by Western blot in the two doxorubicin groups 6,12,18,24, and 48 hours later after the injection. The expressions of HO-1 in liver,spleen and kidney were also detected by immunohistochemistry 48 hours later after the injection and at the same time the indicator of liver function test, serum TNF-α and ET-1 were also determined in the three groups. Results The expression of HO-1 in liver could be detected by Western blot 6 hours later after the injection in the two doxorubicin groups and the expression increased continuously until 24 hours later. The expression of HO-1 was also detected in liver,spleen and kidney by immunohistochemistry 48 hours later after the injection of doxorubicin. The indicator of liver function test,serum TNF-α and ET-1 were much higher in the high-dose doxorubicin group than those in the control group and low-dose doxorubicin group (P<0.05), there was no significant difference between the control and low-dose doxorubicin groups (P>0.05). Conclusion Doxorubicin can induce the expression of HO-1 in liver,spleen and kidney, low-dose doxorubicin may do no harm to liver.

Key words: doxorubicin, heme oxygenase (decyclizing), biosynthesis, liver function

中图分类号:

张海山，张学文，马淑荣，张德恒，邱东涛. 阿霉素诱导亚铁血红素氧化酶-1在大鼠肝、脾和肾脏组织的表达及其对肝功能的影响[J]. J4, 2004, 30(1): 82-84.

ZHANG Hai-shan,ZHANG Xue-wen, MA Shu-rong,ZHANG De-heng，QIU Dong-tao. Expression of heme oxygenase-1 induced by doxorubicin in liver，spleen， and kidney and effect of doxorubicin on liver function[J]. J4, 2004, 30(1): 82-84.

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