关键词: 骨髓基质细胞, 组织工程骨, 骨生成

Abstract: Objective To observe heterotopic osteogenesis of autogenous marrow stromal cells on β-tricalcium phosphate（β-TCP） scaffold,and the effects of recombinant human bone morphogenetic protein-2(rhBMP-2) and transforming growth factor-beta (TGF-β) on osteogenesis. Methods The Newzealand rabbits autogenous marrow stromal cells were cultured by mineralized condition medium. The cell suspension was collected. All the cells with concentration of 5×106·mL^-1 we re divided into four groups. Group A: rhBMP-2（15 μg）/TGF-β（30 ng）were added; group B:TGF-β 30 ng was administered; group C:without growth factor as control; group D:continual cultured and no loading on β-TCP. MSCs in group A,B,C were planted on β-TCP.Alkaline phosphatase (ALP) activity was examined by histochemistry. Then MSCs loading on β-TCP were embedded in autogenous subcutane ous. Four weeks later, the osteogenesis was observed by HE staining, typeⅠcollagen and bone morphogenetic protein synthesis were examined by immunohistochemistry stains. Results The ALP activities in groups A and B were significantly higher than those in group C and D （P<0.01）. Five weeks later, some strip new bone formations were observed by HE staining in all groups, the bone matrix area in group A was significantly bigger than that in group B（P<0.01）. The average gray degree of type Ⅰ collagen in groups A and B were lower than that in group C. There was not obviously difference of bone morphogenetic protein expression between group A, B and C. Conclusion β-TCP and MSCs can promote heterotopic osteogenesis, rhBMP-2 and TGF-β have the stronger effect to enhance osteogenesis than rhBMP-2.

Key words: marrow stromal cells, tissue engineering bone, osteogenesis