神经肽Y在杏仁中央核内对禁食大鼠摄食的调节方式

doi:DOI：10.7694/jldxyxb20130405

吉林大学学报(医学版)

神经肽Y在杏仁中央核内对禁食大鼠摄食的调节方式

朱永香¹，王倩¹，王爽²，于玮¹，南瑛¹，曹健¹

(1. 西安医学院生理学教研室，陕西西安 710021； 2. 西安医学院病理生理学教研室，陕西西安 710021)

收稿日期:2013-01-03 出版日期:2013-07-28 发布日期:2013-07-28
通讯作者: 王爽 E-mail:(Tel：029-86177414，E-mail：wangshuang78213@163.com)
作者简介:朱永香（1977-），女，河南省开封市人，讲师，医学硕士，主要从事味觉和摄食的外周及中枢调控机制研究。
基金资助:
国家自然科学基金资助课题(8110665)；陕西省科技厅自然科学基金资助课题（2011JQ4005）

Regulation of neuropeptide Y in central nucleus of amygdaloid on feeding of fasted rats

ZHU Yong-xiang¹,WANG Qian¹,WANG Shuang²,YU Wei1,NAN Ying¹,CAO Jian¹

(1. Department of Physiology,Xi’an Medical College,Xi’an 710021,China；2. Department of Pathophysiology,Xi’an Medical College,Xi’an 710021,China)

Received:2013-01-03 Online:2013-07-28 Published:2013-07-28

摘要/Abstract

摘要：

目的：观察神经肽Y（NPY）在杏仁中央核（CeA）内对禁食大鼠摄食的调节方式，并探讨其可能的作用机制。方法：选择雄性SD大鼠34只，将其中10只大鼠随机分为正常摄食组与禁食24 h组，每组5只，取大鼠脑组织，应用免疫组织化学方法检测大鼠CeA内NPY及其Y1受体的表达；剩余24只大鼠行CeA内套管植入术，术后12只大鼠随机分为2组，1组CeA内注射NPY，另1组CeA内注射生理盐水(对照1组)，1、2及4 h后分别观察大鼠摄食量；另12只术后大鼠随机分为2组，1组CeA内注射Y1受体阻断剂，另1组CeA内注射生理盐水(对照2组)，1、2 及4 h后分别观察大鼠摄食量。结果：与正常摄食组比较，禁食24 h组大鼠CeA内NPY表达显著增多（P<0.05），其Y1受体表达显著减少（P<0.05）；与对照1组比较，大鼠CeA内注射NPY后1 h内摄食量明显增加（P<0.05）；与对照2组比较，大鼠CeA内注射NPY的Y1受体阻断剂后1 h内摄食量明显减少（P<0.05）。结论：NPY在 CeA内对禁食大鼠的摄食具有促进作用，其机制可能是通过Y1受体调节。

关键词: 神经肽Y, 杏仁中央核, 神经肽Y1受体, 摄食

Abstract:

Abstract:Objective
To examine the regulatory mode of neuropeptide Y (NPY) in the central nucleus of amygdala(CeA) on feeding of fasted rats，and to explore the possible mechanism. Methods 34 male SD rats were chosen,and 10 rats of them were divided into feeding group(n=5) and fasted 24 h group(n=5),and the rat brain was obtained. The expressions of NPY and neuropeptide Y 1 receptor(Y1R)in CeA were detected by immunohistochemical staining method. The other 24 rats were implanted cannula in CeA. The postoperative 12 rats were randomly divided into 2 groups,and the rats in one group were injected with NPY and the rats in another group were injected with saline in CeA(control 1 group),after 1,2 and 4 h,the intake of food was detected. The other postoperative 12 rats were randomly divided into 2 groups,and the rats in one group were injected with Y1R antagonist and the rats in another group were injected with saline in CeA(control 2 group),after 1,2 and 4 h,the intake of food was detected. Results Compared with feeding group,the NPY expression in CeA of the rats in fasted 24 h group was significantly increased (P<0.05),and the Y1R expression in CeA of the rats in fasted 24 h group was significantly decreased (P<0.05). Compared with control 1 group,the intake of food of the rats injected with NPY in CeA within 1 h was significantly increased(P<0.05);compared with control 2 group,the intake of food of the rats injected with Y1R antagonist in CeA within 1 h was significantly decreased(P<0.05). Conclusion NPY in CeA has promotion effect on the feeding of fasted rats,and the mechanism may be regulated by Y1 receptor.


Key words: neuropeptide Y, central nucleus of amygdala, neuropeptide Y1 receptor, feeding

中图分类号:

R338.2

朱永香，王倩，王爽，于玮，南瑛，曹健. 神经肽Y在杏仁中央核内对禁食大鼠摄食的调节方式[J]. 吉林大学学报(医学版), 2013, 39(4): 667-671.

ZHU Yong-xiang,WANG Qian,WANG Shuang,YU Wei,NAN Ying,CAO Jian. Regulation of neuropeptide Y in central nucleus of amygdaloid on feeding of fasted rats[J]. Journal of Jilin University Medicine Edition, 2013, 39(4): 667-671.

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[2]	陈立刚，迟宝荣，孙艳. 神经肽Y的临床研究进展[J]. J4, 2007, 33(2): 399-401.
[3]	纪文新，张远卓，邱杰,秦玲. 神经肽Y与神经降压素改变及比例失调在高血压发病中的作用[J]. J4, 2006, 32(5): 866-868.
[4]	陈立刚，迟宝荣. 血浆神经肽Y水平测定在判定慢性肝脏疾病病变程度及预后中的意义[J]. J4, 2006, 32(1): 113-3.

神经肽Y在杏仁中央核内对禁食大鼠摄食的调节方式

Regulation of neuropeptide Y in central nucleus of amygdaloid on feeding of fasted rats

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